| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSF2RB |
| Uniprot No | P32927 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 17-443aa |
| 氨基酸序列 | WERSLAGAEETIPLQTLRCYNDYTSHITCRWADTQDAQRLVNVTLIRRVNEDLLEPVSCDLSDDMPWSACPHPRCVPRRCVIPCQSFVVTDVDYFSFQPDRPLGTRLTVTLTQHVQPPEPRDLQISTDQDHFLLTWSVALGSPQSHWLSPGDLEFEVVYKRLQDSWEDAAILLSNTSQATLGPEHLMPSSTYVARVRTRLAPGSRLSGRPSKWSPEVCWDSQPGDEAQPQNLECFFDGAAVLSCSWEVRKEVASSVSFGLFYKPSPDAGEEECSPVLREGLGSLHTRHHCQIPVPDPATHGQYIVSVQPRRAEKHIKSSVNIQMAPPSLNVTKDGDSYSLRWETMKMRYEHIDHTFEIQYRKDTATWKDSKTETLQNAHSMALPALEPSTRYWARVRVRTSRTGYNGIWSEWSEARSWDTESVLPMW |
| 预测分子量 | 50.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSF2RB重组蛋白的3篇代表性文献概览：
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1. **文献名称**：*Structural and functional characterization of recombinant human CSF2RB*
**作者**：Smith A, et al.
**摘要**：本研究通过昆虫细胞表达系统成功制备了重组人CSF2RB蛋白，解析其晶体结构并验证其与GM-CSF受体的结合能力，揭示了β共同链在细胞因子信号转导中的构象变化机制。
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2. **文献名称**：*Recombinant CSF2RB enhances hematopoietic progenitor cell proliferation in vitro*
**作者**：Zhang L, et al.
**摘要**：利用哺乳动物细胞表达的重组CSF2RB蛋白，证明其与IL-3受体α链结合后显著促进造血干/祖细胞的体外扩增，为再生医学中的细胞疗法提供了潜在工具。
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3. **文献名称**：*Targeting CSF2RB/JAK2 signaling in myeloid leukemia using recombinant decoy receptors*
**作者**：Kim H, et al.
**摘要**：开发了重组CSF2RB诱饵蛋白，通过阻断GM-CSF和IL-3通路抑制JAK/STAT信号传导，在髓系白血病小鼠模型中显示出抗肿瘤活性，提示其作为靶向治疗药物的潜力。
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注：上述文献为示例性质，实际引用时需根据具体研究内容选择匹配的论文。建议通过PubMed或Web of Science以“CSF2RB recombinant”或“GM-CSF receptor beta subunit”为关键词检索最新文献。
The CSF2RB protein, also known as the β common chain (βc), is a critical component of the receptor complexes for granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), and interleukin-5 (IL-5). It belongs to the type I cytokine receptor family and functions as a shared signaling subunit, pairing with ligand-specific α subunits to form high-affinity receptors. This transmembrane protein plays a vital role in hematopoiesis, immune response regulation, and inflammation by activating downstream signaling pathways, particularly the JAK-STAT, MAPK, and PI3K cascades.
Recombinant CSF2RB proteins are engineered using expression systems like mammalian or insect cells to preserve post-translational modifications and structural integrity. These proteins are essential tools for studying receptor-ligand interactions, signal transduction mechanisms, and dysregulated pathways in diseases such as leukemia, asthma, and autoimmune disorders. In therapeutic research, CSF2RB is investigated for its potential in modulating immune responses—either by blocking aberrant signaling in inflammatory conditions or enhancing anti-tumor immunity.
Structurally, the recombinant protein often includes extracellular domains for binding studies or modified versions (e.g., soluble forms) to interrogate receptor activation. Its role in supporting the survival, proliferation, and differentiation of myeloid cells makes it a target for drug development, including monoclonal antibodies and small-molecule inhibitors. However, challenges remain in balancing therapeutic efficacy with off-target effects due to the shared use of βc across multiple cytokines. Ongoing research aims to delineate precise regulatory mechanisms and explore tissue-specific targeting strategies.
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