| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYTIP |
| Uniprot No | O60759 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-359aa |
| 氨基酸序列 | MSLQRLLQHS SNGNLADFCA GPAYSSYSTL TGSLTMDDNR RIQMLADTVA TLPRGRKQLA LTRSSSLSDF SWSQRKLVTV EKQDNETFGF EIQSYRPQNQ NACSSEMFTL ICKIQEDSPA HCAGLQAGDV LANINGVSTE GFTYKQVVDL IRSSGNLLTI ETLNGTMILK RTELEAKLQV LKQTLKQKWV EYRSLQLQEH RLLHGDAANC PSLENMDLDE LSLFGPLPGP GPALVDRNRL SSESSCKSWL SSMTMDSEDG YQTCVSEDSS RGAFSRQTST DDECFIPKEG DDFLRRSSSR RNRSISNTSS GSMSPLWEGN LSSMFGTLPR KSRKGSVRKQ LLKFIPGLHR AVEEEESRF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYTIP重组蛋白的3篇参考文献概览(基于已有研究整合，非真实文献引用)：
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1. **标题**：CYTIP regulates T cell β2-integrin signaling and immune synapse formation
**作者**：Müller, T. et al.
**摘要**：研究揭示了CYTIP通过与Cytohesin-1相互作用调控T细胞中β2整合素信号通路，促进LFA-1介导的细胞黏附和免疫突触形成。重组蛋白实验表明CYTIP缺失导致T细胞迁移缺陷。
2. **标题**：Structural and functional analysis of CYTIP in dendritic cell migration
**作者**：Staiger, H. et al.
**摘要**：通过重组CYTIP蛋白及突变体实验，证明其PH结构域对树突状细胞趋化因子响应至关重要，调控细胞极性及淋巴结归巢能力，为免疫应答提供分子机制依据。
3. **标题**：CYTIP knockout mice exhibit impaired macrophage adhesion and phagocytosis
**作者**：Weber, K.S. et al.
**摘要**：利用重组蛋白回补实验，发现CYTIP缺失巨噬细胞中整合素活化受阻，导致病原体吞噬功能下降，证实CYTIP在先天免疫中的关键作用。
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**注**：以上为模拟文献，实际文献需通过PubMed等数据库检索关键词“CYTIP recombinant protein”或结合具体研究领域筛选。
Cytip (cytohesin interacting protein), also known as PSCDIBP or cytohesin-1-interacting protein, is a cytoplasmic protein that plays a regulatory role in cellular adhesion, migration, and immune response signaling. It was initially identified as an interacting partner of cytohesins, a family of guanine nucleotide exchange factors (GEFs) involved in small GTPase signaling. Structurally, Cytip contains a coiled-coil domain critical for mediating protein-protein interactions, particularly with cytohesin-1 and components of the β1-integrin activation pathway.
In immune cells, Cytip regulates integrin-mediated adhesion by modulating cytohesin-1 activity, thereby influencing T-cell receptor signaling, dendritic cell migration, and immunological synapse formation. Its interaction with cytohesin-1 disrupts the recruitment of ARF GTPases, which are essential for cytoskeletal reorganization and membrane trafficking. Dysregulation of Cytip has been implicated in inflammatory diseases and cancer progression, with studies showing altered expression in leukemia and solid tumors.
Recombinant Cytip protein is typically produced using bacterial or mammalian expression systems to ensure proper folding and post-translational modifications. It serves as a vital tool for studying integrin signaling pathways, immune cell dynamics, and molecular mechanisms underlying metastasis. Purification methods often involve affinity tags (e.g., His-tag) followed by chromatography. Research applications include protein interaction assays, cell migration studies, and therapeutic target validation. As an emerging player in cellular communication, recombinant Cytip continues to facilitate discoveries in immunology, oncology, and cell adhesion biology.
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