| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYTH2 |
| Uniprot No | Q99418-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-399aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMEDGVYE PPDLTPEERM ELENIRRRKQ ELLVEIQRLR EELSEAMSEV EGLEANEGSK TLQRNRKMAM GRKKFNMDPK KGIQFLVENE LLQNTPEEIA RFLYKGEGLN KTAIGDYLGE REELNLAVLH AFVDLHEFTD LNLVQALRQF LWSFRLPGEA QKIDRMMEAF AQRYCLCNPG VFQSTDTCYV LSFAVIMLNT SLHNPNVRDK PGLERFVAMN RGINEGGDLP EELLRNLYDS IRNEPFKIPE DDGNDLTHTF FNPDREGWLL KLGGRVKTWK RRWFILTDNC LYYFEYTTDK EPRGIIPLEN LSIREVDDPR KPNCFELYIP NNKGQLIKAC KTEADGRVVE GNHMVYRISA PTQEEKDEWI KSIQAAVSVD PFYEMLAARK KRISVKKKQE QP |
| 预测分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYTH2重组蛋白的3篇代表性文献的简要概括(注：文献为示例性内容，实际引用需核实具体数据库)：
---
1. **文献名称**: "Structural and functional characterization of the CYTH2 recombinant protein as an Arf6 GTPase activator"
**作者**: Smith J. et al.
**摘要**: 本研究通过重组表达纯化CYTH2蛋白，解析其晶体结构，揭示了其与Arf6结合的分子机制。实验表明CYTH2通过激活Arf6 GTPase活性调控细胞膜运输过程，为理解细胞骨架重塑提供依据。
2. **文献名称**: "CYTH2 regulates cell migration via interaction with β-arrestin in cancer cells"
**作者**: Li X. et al.
**摘要**: 文章利用重组CYTH2蛋白进行体外互作实验，发现CYTH2通过结合β-arrestin调控EGFR信号通路，促进肿瘤细胞迁移。研究为靶向CYTH2的抗癌药物开发奠定基础。
3. **文献名称**: "Expression optimization and functional analysis of recombinant CYTH2 in neurodegenerative models"
**作者**: Garcia R. et al.
**摘要**: 作者优化了CYTH2重组蛋白在大肠杆菌中的表达条件，并证明其在神经元模型中通过调节突触囊泡运输减缓神经退行性病变，提示其潜在治疗价值。
---
如需具体文献，建议通过PubMed或Google Scholar检索关键词“CYTH2 recombinant protein”“CYTH2 Arf GEF”等获取最新研究。
CYTH2. also known as ARNO (ADP-ribosylation factor nucleotide-binding site opener), is a member of the cytohesin family of proteins involved in cellular signaling and membrane trafficking. It functions as a guanine nucleotide exchange factor (GEF) for ADP-ribosylation factors (ARFs), small GTPases that regulate vesicular transport, cytoskeletal reorganization, and receptor signaling. CYTH2 specifically activates ARF1 and ARF6 by catalyzing the exchange of GDP for GTP, thereby modulating downstream pathways critical for processes like endocytosis, cell adhesion, and immune response. Its structure includes a coiled-coil domain, a Sec7 domain (responsible for GEF activity), and a pleckstrin homology (PH) domain that binds phosphoinositides, linking it to membrane interactions.
Recombinant CYTH2 protein is engineered for experimental studies to elucidate its molecular mechanisms and interactions. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant form often includes tags (e.g., His, GST) for purification and detection. Researchers use it to investigate its role in cellular trafficking, signal transduction, and disease contexts. For instance, CYTH2 dysregulation has been implicated in cancer metastasis, neurodevelopmental disorders, and immune dysfunction. Its involvement in ARF-mediated pathways also makes it a potential therapeutic target. Studies with recombinant CYTH2 have advanced understanding of how GEFs spatially and temporally control ARF activation, offering insights into membrane dynamics and intracellular communication. This protein remains a key tool for dissecting the complexity of ARF signaling networks in health and disease.
×
