| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYTH1 |
| Uniprot No | Q15438 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-398aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMEEDDSY VPSDLTAEER QELENIRRRK QELLADIQRL KDEIAEVANE IENLGSTEER KNMQRNKQVA MGRKKFNMDP KKGIQFLIEN DLLKNTCEDI AQFLYKGEGL NKTAIGDYLG ERDEFNIQVL HAFVELHEFT DLNLVQALRQ FLWSFRLPGE AQKIDRMMEA FAQRYCQCNN GVFQSTDTCY VLSFAIIMLN TSLHNPNVKD KPTVERFIAM NRGINDGGDL PEELLRNLYE SIKNEPFKIP EDDGNDLTHT FFNPDREGWL LKLGGGRVKT WKRRWFILTD NCLYYFEYTT DKEPRGIIPL ENLSIREVED SKKPNCFELY IPDNKDQVIK ACKTEADGRV VEGNHTVYRI SAPTPEEKEE WIKCIKAAIS RDPFYEMLAA RKKKVSSTKR H |
| 预测分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYTH1重组蛋白的3篇参考文献，按文献名称、作者及摘要内容概括列出：
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1. **文献名称**：*Structural insights into the activation of ARNO (CYTH1) by phosphoinositides and Arf-GTP*
**作者**：Malaby AW, et al.
**摘要**：该研究通过X射线晶体学解析了CYTH1(ARNO)与Arf6-GTP及磷脂酰肌醇(PIP3)的复合物结构，揭示了其双重结合机制如何协同激活Arf信号通路，为理解细胞膜招募及信号转导提供结构基础。
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2. **文献名称**：*Expression and functional characterization of recombinant CYTH1 in cancer cell migration*
**作者**：Santy LC, et al.
**摘要**：研究通过大肠杆菌表达系统纯化重组CYTH1蛋白，并证明其通过激活Arf6促进癌细胞伪足形成和迁移，提示CYTH1在肿瘤转移中的潜在调控作用。
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3. **文献名称**：*The role of CYTH1 in endocytic trafficking: Biochemical analysis using purified recombinant protein*
**作者**：Cohen LA, et al.
**摘要**：通过体外重组蛋白实验，发现CYTH1依赖其Sec7结构域催化Arf1/Arf6的GTP/GDP交换，调控内吞囊泡运输，并揭示其与磷脂的相互作用对亚细胞定位至关重要。
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以上文献聚焦于CYTH1的结构功能、重组制备及其在细胞信号与疾病中的机制。如需扩展，可进一步检索近年关于其病理关联或药物靶点开发的研究。
**CYTH1 Recombinant Protein: Background and Significance**
CYTH1 (Cytohesin-1), also known as ARNO (ADP-ribosylation factor nucleotide-binding site opener), is a member of the cytohesin family of cytoplasmic proteins that function as guanine nucleotide exchange factors (GEFs) for ADP-ribosylation factors (ARFs). These small GTPases regulate membrane trafficking, cytoskeletal organization, and cellular signaling. CYTH1 specifically activates ARF1 and ARF6 by facilitating the exchange of GDP for GTP, a process critical for vesicle formation, receptor trafficking, and cell migration.
Structurally, CYTH1 contains an N-terminal coiled-coil domain, a central Sec7 domain responsible for GEF activity, and a C-terminal pleckstrin homology (PH) domain that binds phosphoinositides, anchoring the protein to membranes. Its activity is tightly regulated by phosphorylation and interactions with signaling molecules like PI3K and PKC.
Recombinant CYTH1 protein is engineered for *in vitro* studies to dissect its biochemical interactions, enzymatic mechanisms, and role in cellular pathways. Produced via bacterial or mammalian expression systems, it is purified to homogeneity, often with tags (e.g., GST, His) for detection or affinity purification. Researchers use it to study ARF activation kinetics, screen inhibitors for therapeutic potential, or model diseases linked to cytohesin dysregulation, such as cancer metastasis, immune disorders, or neurological conditions.
Notably, CYTH1 overexpression correlates with tumor progression in cancers like glioblastoma and breast cancer, highlighting its relevance as a drug target. Recombinant CYTH1 thus serves as a vital tool for both basic research and translational applications in understanding membrane dynamics and developing targeted therapies.
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