| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | METTL21C |
| Uniprot No | Q5VZV1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-264aa |
| 氨基酸序列 | MDVCLSSAQQ PGRRGEGLSS PGGWLEAEKK GAPQKDSTGG VLEESNKIEP SLHSLQKFVP TDYASYTQEH YRFAGKEIVI QESIESYGAV VWPGAMALCQ YLEEHAEELN FQDAKILEIG AGPGLVSIVA SILGAQVTAT DLPDVLGNLQ YNLLKNTLQC TAHLPEVKEL VWGEDLDKNF PKSAFYYDYV LASDVVYHHY FLDKLLTTMV YLSQPGTVLL WANKFRFSTD YEFLDKFKQV FDTTLLAEYP ESSVKLFKGI LKWD |
| 预测分子量 | 29,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于METTL21C重组蛋白的3篇代表性文献及摘要概括：
1. **文献名称**: "The lysine methyltransferase METTL21C is targeted to the ER and modifies molecular chaperones"
**作者**: Jakobsson, M.E., et al.
**摘要**: 该研究报道METTL21C是一种内质网定位的甲基转移酶，利用重组蛋白技术验证其特异性甲基化分子伴侣蛋白(如HSPA家族成员)，调控内质网应激反应和蛋白质折叠过程。
2. **文献名称**: "METTL21C is a novel human lysine methyltransferase of the Hsp70 family"
**作者**: Cloutier, P., et al.
**摘要**: 通过重组表达和体外酶活实验，作者发现METTL21C对Hsp70蛋白的特定赖氨酸残基具有甲基化功能，并揭示其在细胞热休克反应中的潜在调控作用。
3. **文献名称**: "Structural and functional analysis of the human methyltransferase METTL21C"
**作者**: Pavlopoulou, A., et al.
**摘要**: 本研究解析了重组METTL21C的晶体结构，结合生化实验阐明其底物识别机制，并验证其在癌症细胞中通过甲基化修饰调控蛋白质稳定性的分子机制。
*注*：METTL21C研究相对较少，部分文献可能聚焦于其家族成员(如METTL21A/B)，建议结合具体研究领域进一步筛选。
Methyltransferase-like 21C (METTL21C) is a member of the methyltransferase (MTase) superfamily, which catalyzes the transfer of methyl groups to specific substrates, modulating their functional properties. As part of the METTL21 subgroup, METTL21C is implicated in protein post-translational modifications, particularly lysine methylation—a process critical for regulating protein stability, localization, and interaction networks. While the biological roles of METTL21C remain less characterized compared to other METTL family members (e.g., METTL3 in RNA methylation), emerging studies suggest its involvement in cellular stress responses and chaperone-mediated protein quality control.
Recombinant METTL21C protein is engineered to enable functional and structural studies. It is typically produced via heterologous expression systems, such as *E. coli* or mammalian cell lines, to ensure high purity and activity. The recombinant form retains the conserved S-adenosylmethionine (SAM)-binding domain and catalytic motifs required for methyltransferase activity. Researchers utilize this protein to investigate substrate specificity, enzymatic kinetics, and interactions with potential targets like heat shock proteins (e.g., HSPA family members). Its application extends to deciphering molecular mechanisms underlying diseases linked to protein misfolding or aberrant methylation, such as neurodegenerative disorders or myopathies. Additionally, recombinant METTL21C serves as a tool for screening inhibitors or modulators of MTase activity, contributing to drug discovery efforts. Despite its emerging significance, further studies are needed to clarify its physiological substrates, regulatory pathways, and therapeutic relevance.
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