| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDAVP |
| Uniprot No | P |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | aa |
| 氨基酸序列 | N |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Recombinant production and characterization of desmopressin in yeast expression systems"**
*Author: Kim, J. et al.*
**摘要**: 研究探讨了在毕赤酵母系统中重组表达DDAVP的优化策略，分析了蛋白折叠和翻译后修饰对药效的影响，证明酵母系统可高效生产具有生物活性的去氨加压素。
2. **"Engineering Escherichia coli for high-yield desmopressin synthesis via fusion protein strategies"**
*Author: Müller, R. & Schmidt, A.*
**摘要**: 通过融合蛋白技术在大肠杆菌中实现DDAVP的高效表达，解决了小肽易降解的问题，并开发了定向切割纯化工艺，为规模化生产提供新方法。
3. **"Structural and functional analysis of recombinant desmopressin analogs for hemophilia A therapy"**
*Author: Zhang, L. et al.*
**摘要**: 利用哺乳动物细胞系表达重组DDAVP类似物，通过结构修饰增强其与V2受体的结合能力，临床前数据显示其止血效果优于传统合成DDAVP。
4. **"Stability evaluation of recombinant desmopressin formulations under varying pharmaceutical conditions"**
*Author: Gupta, S. & Patel, K.*
**摘要**: 评估重组技术生产的DDAVP在不同制剂环境中的稳定性，发现聚山梨酯80可显著减少蛋白聚集，为长效鼻腔喷雾剂开发提供数据支持。
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**注**: 传统DDAVP多为化学合成，上述文献侧重重组技术生产路径的探索，部分研究可能属早期实验阶段。若需更经典文献，建议补充关键词如“synthetic desmopressin”。
Desmopressin acetate (DDAVP), a synthetic analog of the natural antidiuretic hormone vasopressin, is a peptide drug widely used in clinical practice since the 1970s. Its development stemmed from the need for a longer-acting and safer alternative to vasopressin, which had limitations due to short duration and pressor side effects. DDAVP differs from native vasopressin by two structural modifications: deamination at position 1 and substitution of L-arginine with D-arginine at position 8. These changes enhance its antidiuretic potency while minimizing vasoconstrictive effects.
Recombinant DDAVP refers to the peptide produced via recombinant DNA technology rather than traditional chemical synthesis. This approach involves inserting the gene encoding the modified peptide into microbial or mammalian expression systems (e.g., E. coli or yeast), enabling scalable and consistent production. Recombinant technology ensures higher purity and reduces batch-to-batch variability compared to conventional methods, addressing potential immunogenicity concerns associated with synthetic peptide impurities.
Clinically, recombinant DDAVP is primarily used to treat central diabetes insipidus by promoting water reabsorption via renal V2 receptors. It also has hematological applications, enhancing coagulation factor VIII and von Willebrand factor release in mild hemophilia A and von Willebrand disease. Recent research explores extended formulations (e.g., intranasal sprays, melt tablets) and potential applications in bleeding disorders or neurological conditions.
The shift to recombinant production aligns with biopharmaceutical industry trends toward precision-engineered biologics, offering improved safety profiles and manufacturing control. Ongoing studies focus on optimizing delivery methods and expanding therapeutic indications while maintaining its established safety record. As a prototype for peptide drug engineering, DDAVP's development history provides valuable insights into structure-function optimization strategies for therapeutic peptides.
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