| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CYB5R2 |
| Uniprot No | Q6BCY4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-276aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMNSRRRE PITLQDPEAK YPLPLIEKEK ISHNTRRFRF GLPSPDHVLG LPVGNYVQLL AKIDNELVVR AYTPVSSDDD RGFVDLIIKI YFKNVHPQYP EGGKMTQYLE NMKIGETIFF RGPRGRLFYH GPGNLGIRPD QTSEPKKTLA DHLGMIAGGT GITPMLQLIR HITKDPSDRT RMSLIFANQT EEDILVRKEL EEIARTHPDQ FNLWYTLDRP PIGWKYSSGF VTADMIKEHL PPPAKSTLIL VCGPPPLIQT AAHPNLEKLG YTQDMIFTY |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CYB5R2重组蛋白的3篇代表性文献的概括(内容基于公开研究主题综合整理，具体文献需以实际检索结果为准)：
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1. **文献名称**：*"Characterization of human CYB5R2 recombinant protein: Role in lipid metabolism and oxidative stress"*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌表达系统纯化重组CYB5R2蛋白，验证其NADH依赖性还原酶活性，发现其参与细胞脂质去饱和过程，并可能通过调节脂筏氧化状态影响细胞应激反应。
2. **文献名称**：*"Structural insights into CYB5R2 by X-ray crystallography: Implications for electron transfer mechanisms"*
**作者**：Li J, et al.
**摘要**：首次解析CYB5R2重组蛋白的晶体结构(2.1 Å分辨率)，揭示其与辅酶FAD结合的活性位点特征，提出其电子传递路径模型，为靶向药物设计提供结构基础。
3. **文献名称**：*"CYB5R2 knockdown exacerbates hepatic steatosis via impaired VLDL assembly in vitro"*
**作者**：Garcia-Ruiz C, et al.
**摘要**：利用重组CYB5R2蛋白及siRNA技术，证明其在肝细胞中通过调节微粒体脂肪酸延伸酶活性影响VLDL分泌，缺失导致甘油三酯蓄积，提示与脂肪肝病理相关。
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注：以上为模拟摘要，实际文献需通过PubMed/Google Scholar检索关键词“CYB5R2 recombinant”“NADH-cytochrome b5 reductase 2”等获取。
CYB5R2 (Cytochrome b5 Reductase 2) is a member of the NADH-cytochrome b5 reductase family, which plays a critical role in electron transfer reactions within cellular metabolism. This flavoprotein catalyzes the reduction of cytochrome b5 using NADH as an electron donor, facilitating processes such as fatty acid desaturation, cholesterol biosynthesis, and drug metabolism. The enzyme exists in both membrane-bound and soluble isoforms, with CYB5R2 primarily localized to the endoplasmic reticulum and mitochondria. Its structure includes a conserved FAD-binding domain and a catalytic site critical for its oxidoreductase activity.
Recombinant CYB5R2 protein is produced through genetic engineering techniques, often expressed in bacterial or mammalian systems to study its biochemical properties and interactions. Researchers utilize this purified protein to investigate its enzymatic kinetics, substrate specificity, and regulatory mechanisms. Studies have linked CYB5R2 dysfunction to metabolic disorders, cancer progression, and neurodegenerative diseases, underscoring its physiological relevance. For instance, altered CYB5R2 expression has been observed in certain tumors, suggesting a potential role in redox homeostasis and cancer cell survival. Additionally, its involvement in lipid metabolism highlights connections to cardiovascular and metabolic syndromes.
Recent advances in structural biology using recombinant CYB5R2 have provided insights into its conformational dynamics and interaction partners, aiding drug discovery efforts targeting metabolic pathways. Its application extends to industrial biocatalysis for synthesizing bioactive compounds. Despite progress, questions remain about tissue-specific regulation and post-translational modifications, driving ongoing research to elucidate its full functional spectrum. The development of recombinant CYB5R2 continues to be vital for both basic science and therapeutic innovation.
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