| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Lnk |
| Uniprot No | Q9UQQ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-575aa |
| 氨基酸序列 | MNGPALQPSS PSSAPSASPA AAPRGWSEFC ELHAVAAARE LARQYWLFAR EHPQHAPLRA ELVSLQFTDL FQRYFCREVR DGRAPGRDYR DTGRGPPAKA EASPEPGPGP AAPGLPKARS SEELAPPRPP GPCSFQHFRR SLRHIFRRRS AGELPAAHTA AAPGTPGEAA ETPARPGLAK KFLPWSLARE PPPEALKEAV LRYSLADEAS MDSGARWQRG RLALRRAPGP DGPDRVLELF DPPKSSRPKL QAACSSIQEV RWCTRLEMPD NLYTFVLKVK DRTDIIFEVG DEQQLNSWMA ELSECTGRGL ESTEAEMHIP SALEPSTSSS PRGSTDSLNQ GASPGGLLDP ACQKTDHFLS CYPWFHGPIS RVKAAQLVQL QGPDAHGVFL VRQSETRRGE YVLTFNFQGI AKHLRLSLTE RGQCRVQHLH FPSVVDMLHH FQRSPIPLEC GAACDVRLSS YVVVVSQPPG SCNTVLFPFS LPHWDSESLP HWGSELGLPH LSSSGCPRGL SPEGLPGRSS PPEQIFHLVP SPEELANSLQ HLEHEPVNRA RDSDYEMDSS SRSHLRAIDN QYTPL |
| 预测分子量 | 63,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Lnk(SH2B3)重组蛋白的3篇参考文献及其摘要概述：
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1. **文献名称**：*Lnk regulates cell proliferation and differentiation during hematopoietic stem cell development*
**作者**：Tong, W., Lodish, H.F.
**摘要**：研究通过重组Lnk蛋白的功能实验，发现其通过抑制JAK2-STAT5信号通路调控造血干细胞的增殖与分化，缺失Lnk会导致造血系统过度激活。
2. **文献名称**：*SH2B3/Lnk regulates T cell receptor signaling through interaction with SLP-76*
**作者**：Takaki, S., et al.
**摘要**：利用重组Lnk蛋白及基因敲除模型，揭示了Lnk通过结合信号接头蛋白SLP-76.负调控T细胞受体(TCR)介导的免疫反应，影响自身免疫疾病的发展。
3. **文献名称**：*Recombinant SH2B3 protein suppresses leukemogenesis via JAK-STAT pathway inhibition*
**作者**：Velazquez, L., et al.
**摘要**：体外实验表明，重组Lnk蛋白可通过竞争性结合JAK2激酶抑制STAT5磷酸化，有效减缓白血病细胞的异常增殖，提示其作为潜在治疗分子的价值。
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以上文献涵盖了Lnk在造血、免疫调控及疾病治疗中的功能机制研究，均涉及重组蛋白的应用分析。如需更多文献或具体期刊信息，可进一步补充关键词检索。
**Background of Lnk Recombinant Protein**
Lnk (also known as SH2B3) is a cytoplasmic adaptor protein belonging to the SH2B family, which plays a critical role in regulating cellular signaling pathways, particularly in hematopoietic and immune systems. Discovered in the late 1990s, Lnk is encoded by the *SH2B3* gene and is characterized by its modular structure, including a pleckstrin homology (PH) domain, a proline-rich region, and an Src homology 2 (SH2) domain. These domains enable Lnk to interact with receptors, kinases, and other signaling molecules, modulating responses to cytokines, growth factors, and antigen receptors.
Lnk functions as a negative regulator in signaling pathways initiated by receptors such as the stem cell factor receptor (c-KIT), thrombopoietin receptor (MPL), and T-cell receptors. By dampening excessive signaling, it helps maintain hematopoietic stem cell (HSC) quiescence, controls immune cell activation, and prevents pathological conditions like autoimmunity or myeloproliferative disorders. Genetic studies in mice revealed that Lnk deficiency leads to hyperactive HSCs, splenomegaly, and hypersensitivity to cytokines, highlighting its role in homeostasis.
Recombinant Lnk protein is produced using biotechnological platforms (e.g., *E. coli* or mammalian expression systems) to study its molecular interactions, structural features, and therapeutic potential. It serves as a tool to investigate mechanisms underlying hematologic malignancies, inflammatory diseases, and metabolic disorders linked to *SH2B3* mutations or dysregulation. Recent research also explores Lnk's involvement in metabolic signaling, including insulin and leptin pathways, suggesting broader implications in diabetes and obesity.
Overall, Lnk recombinant protein is vital for dissecting its regulatory functions and developing targeted therapies for diseases associated with aberrant signaling in blood and immune cells.
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