| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CXADR |
| Uniprot No | P78310 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-237aa |
| 氨基酸序列 | LSITTPEEMIEKAKGETAYLPCKFTLSPEDQGPLDIEWLISPADNQKVDQ VIILYSGDKI YDDYYPDLKGRVHFTSNDLKSGDASINVTNLQLSDIGT YQCKVKKAPGVANKKIHLVVLV KPSGARCYVDGSEEIGSDFKIKCEPK EGSLPLQYEWQKLSDSQKMPTSWLAEMTSSVISV KNASSEYSGTYSCT VRNRVGSDQCLLRLNVVPPSNKAG |
| 预测分子量 | 8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CXADR重组蛋白的3篇参考文献信息：
1. **文献名称**：Structural analysis of recombinant CXADR protein in virus entry
**作者**：Smith A, et al.
**摘要**：通过X射线晶体学解析了重组CXADR蛋白的胞外结构域，揭示了其与腺病毒纤维蛋白结合的分子机制，为抗病毒药物设计提供结构基础。
2. **文献名称**：Expression and purification of functional CXADR extracellular domain in mammalian cells
**作者**：Wang Y, et al.
**摘要**：开发了哺乳动物细胞表达系统高效制备功能性CXADR重组蛋白，验证其与柯萨奇病毒的结合活性，为疫苗研发提供可靠抗原来源。
3. **文献名称**：CXADR-Fc融合蛋白抑制肠道病毒71型感染的实验研究
**作者**：Li X, et al.
**摘要**：构建CXADR-Fc重组融合蛋白，证实其通过阻断病毒吸附显著抑制EV71对宿主细胞的感染，提示其作为治疗性生物制剂的潜力。
注：以上文献信息为示例性质，实际引用时建议通过PubMed/Google Scholar检索最新文献，以真实发表内容为准。
**Background of CXADR Recombinant Protein**
CXADR (Coxsackievirus and Adenovirus Receptor) is a transmembrane protein that serves as a primary receptor for adenoviruses and coxsackieviruses, facilitating their entry into host cells. Initially identified for its role in viral pathogenesis, CXADR is now recognized as a multifunctional cell adhesion molecule involved in intercellular junction formation, immune modulation, and tissue homeostasis. It belongs to the immunoglobulin superfamily, featuring extracellular immunoglobulin-like domains, a single-pass transmembrane region, and a cytoplasmic tail implicated in signaling.
Recombinant CXADR protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional forms of the protein for research and therapeutic applications. This recombinant form typically retains key structural domains required for ligand binding, such as the D1 domain critical for adenovirus interaction. Its production enables studies on virus-host interactions, cell adhesion mechanisms, and immune responses.
In biomedical research, CXADR recombinant protein is utilized to block viral infection in vitro, study cell-cell communication in tissues like the heart and brain, and explore its tumor-suppressive roles in cancers where CXADR expression is downregulated. Additionally, it serves as a tool for structural biology (e.g., crystallography) to map binding interfaces for drug design.
Quality-controlled batches are validated via SDS-PAGE, Western blotting, and functional assays (e.g., virus neutralization). As a non-infectious reagent, recombinant CXADR offers a safe platform for developing antiviral strategies, understanding tissue development, and designing targeted therapies. Its dual role in virology and cellular biology underscores its significance in both basic and translational research.
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