| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DIXDC1 |
| Uniprot No | Q155Q3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-683aa |
| 氨基酸序列 | MLACLTRGNL LDVLQEGFNE QQLQAYVAWV NAQLKKRPAV KPVQDLRQDL RDGVILAYLI EIVAGEKLSG VQLSPGNQQE MKNNVEKVLQ FVASKKIRMH QTSAKDIVDG NLKSIMRLVL ALAAHFKPGS SRTVNQGRDS RAPLQSHRPH CATAVAQGAA AALADVCHDM SRSGRDVFRY RQRNSSMDEE IENPYWSVRA LVQQYEGQQR SPSESSCSSL TSPSPIHSAK SESIITQSEE KADFVIIPAE GIENRTEGTD SPLSRDWRPG SPGTYLETSW EEQLLEQQEY LEKEMEEAKK MISGLQALLL NGSLPEDEQE RPLALCEPGV NPEEQLIIIQ SRLDQSMEEN QDLKKELLKC KQEARNLQGI KDALQQRLTQ QDTSVLQLKQ ELLRANMDKD ELHNQNVDLQ RKLDERNRLL GEYKKELGQK DRLLQQHQAK LEEALRKLSD VSYHQVDLER ELEHKDVLLA HCMKREADEA TNYNSHNSQS NGFLLPTAGK GATSVSNRGT SDLQLVRDAL RSLRNSFSGH DPQHHTIDSL EQGISSLMER LHVMETQKKQ ERKVRVKSPR TQVGSEYRES WPPNSKLPHS QSSPTVSSTC TKVLYFTDRS LTPFMVNIPK RLEEVTLKDF KAAIDREGNH RYHFKALDPE FGTVKEEIFH DDDAIPGWEG KIVAWVEEDH GEN |
| 预测分子量 | 77,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DIXDC1重组蛋白的3篇参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*DIXDC1 Phosphorylation and Interaction with C-Jun Promote Glioblastoma Cell Invasion*
**作者**：Smith J, et al. (2018)
**摘要**：研究揭示了DIXDC1重组蛋白在胶质母细胞瘤中的磷酸化机制，发现其通过结合c-Jun激活下游信号通路，促进肿瘤细胞侵袭。重组DIXDC1被用于体外激酶实验和蛋白质互作验证。
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2. **文献名称**：*Structural Insights into DIXDC1 Isoforms and Their Role in Wnt Signaling Regulation*
**作者**：Lee S, Kim D (2020)
**摘要**：通过重组表达DIXDC1两种剪接异构体，解析了其DIX结构域的晶体结构，揭示了异构体差异对Wnt/β-catenin信号通路的调控作用，为靶向治疗提供结构基础。
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3. **文献名称**：*DIXDC1 Recombinant Protein Modulates Actin Cytoskeleton Dynamics in Neural Development*
**作者**：Brown R, et al. (2019)
**摘要**：利用重组DIXDC1蛋白进行体外神经元培养实验，证明其通过结合Rac1调控肌动蛋白重塑，影响神经突触生长和发育，提示其在神经疾病中的潜在应用。
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4. **文献名称**：*A Novel Role of DIXDC1 in Suppressing Colorectal Cancer Metastasis via Integrin Signaling*
**作者**：Zhang Y, et al. (2021)
**摘要**：通过纯化重组DIXDC1蛋白，发现其抑制结直肠癌转移的机制与整合素-FAK信号通路相关，并证明其C端结构域是功能关键区域。
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**备注**：以上文献信息为示例，实际文献需通过PubMed或Google Scholar检索确认。建议使用关键词“DIXDC1 recombinant protein”或“DIXDC1 purification”获取具体文章。
**Background of DIXDC1 Recombinant Protein**
DIXDC1 (Dishevelled-Axin Domain Containing 1) is a cytosolic protein belonging to the DIX domain-containing family, characterized by a conserved N-terminal DIX domain shared with Dishevelled (Dvl) and Axin proteins, which are critical regulators of the Wnt/β-catenin signaling pathway. DIXDC1 plays a multifaceted role in cellular processes, including cell adhesion, migration, and proliferation, by interacting with cytoskeletal components and signaling molecules. It is implicated in modulating both canonical and non-canonical Wnt signaling pathways, potentially acting as a scaffold or adaptor protein to influence signal transduction dynamics.
Studies suggest DIXDC1's involvement in cancer progression, though its role appears context-dependent. In some cancers, such as glioblastoma and colorectal cancer, DIXDC1 is reported to promote tumorigenesis by enhancing cell invasion and metastasis through interactions with focal adhesion kinases (FAK) or integrin signaling. Conversely, in other contexts, it may act as a tumor suppressor by stabilizing adherens junctions and suppressing epithelial-mesenchymal transition (EMT). Beyond oncology, DIXDC1 is linked to neurodevelopmental disorders, as it regulates neuronal migration and dendritic spine formation, with mutations associated with autism spectrum disorders and intellectual disability.
The recombinant DIXDC1 protein, typically produced in *E. coli* or mammalian expression systems, retains functional domains necessary for biochemical and cellular studies. It serves as a vital tool for elucidating molecular interactions, structural analyses, and screening therapeutic agents targeting DIXDC1-related pathways. Its applications extend to understanding tissue-specific signaling crosstalk and developing strategies for diseases linked to Wnt dysregulation or cytoskeletal dysfunction.
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