| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IkBb |
| Uniprot No | Q15653 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-356aa |
| 氨基酸序列 | MAGVACLGKA ADADEWCDSG LGSLGPDAAA PGGPGLGAEL GPGLSWAPLV FGYVTEDGDT ALHLAVIHQH EPFLDFLLGF SAGTEYMDLQ NDLGQTALHL AAILGETSTV EKLYAAGAGL CVAERRGHTA LHLACRVGAH ACARALLQPR PRRPREAPDT YLAQGPDRTP DTNHTPVALY PDSDLEKEEE ESEEDWKLQL EAENYEGHTP LHVAVIHKDV EMVRLLRDAG ADLDKPEPTC GRSPLHLAVE AQAADVLELL LRAGANPAAR MYGGRTPLGS AMLRPNPILA RLLRAHGAPE PEGEDEKSGP CSSSSDSDSG DEGDEYDDIV VHSSRSQTRL PPTPASKPLP DDPRPV |
| 预测分子量 | 37,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IkBβ(NFKBIB)重组蛋白的3篇代表性文献的简要整理(注：内容基于领域内常见研究方向模拟，实际文献请通过学术数据库核实)：
1. **《Functional characterization of recombinant IκBβ expressed in E. coli: phosphorylation-induced degradation and NF-κB inhibition》**
- 作者：Thompson JE, et al.
- 摘要：研究通过大肠杆菌系统表达并纯化重组IκBβ蛋白，证实其可特异性结合NF-κB二聚体(如p50/p65)。实验显示IκBβ在体外可被IKK复合体磷酸化，导致其泛素化降解，从而释放NF-κB并激活下游基因转录。
2. **《Structural insights into IκBβ/NF-κB complex formation through crystallographic analysis of recombinant proteins》**
- 作者：Jacobs MD, Harrison SC.
- 摘要：利用重组IκBβ和p65/p50蛋白进行共结晶，解析了IκBβ的ANK重复结构域与NF-κB的结合模式，揭示了其通过N端区域竞争性阻断NF-κB核定位信号的分子机制。
3. **《IκBβ recombinant protein attenuates LPS-induced septic shock by modulating NF-κB and MAPK pathways in murine models》**
- 作者：Yamamoto M, et al.
- 摘要：通过哺乳动物细胞表达系统制备高纯度IκBβ重组蛋白，发现腹腔注射可显著抑制LPS诱导的小鼠全身炎症反应。机制涉及抑制NF-κB核转位及下调IL-6、TNF-α表达，同时部分调节JNK磷酸化水平。
注：以上为模拟摘要，实际文献请检索PubMed等平台(关键词：NFKBIB, recombinant IκBβ, NF-kappaB inhibition)。
**Background of IkBβ Recombinant Protein**
The inhibitor of nuclear factor kappa B (IkB) family comprises key regulatory proteins that control the activity of NF-κB, a transcription factor central to immune responses, inflammation, and cell survival. Among these, IkBβ (encoded by the *NFKBIB* gene) functions as a critical modulator by binding to NF-κB dimers (e.g., p50/p65), sequestering them in the cytoplasm and preventing their nuclear translocation. Unlike its homolog IkBα, which is rapidly degraded to enable acute NF-κB activation, IkBβ exhibits distinct kinetics and regulatory roles. It forms stable complexes with NF-κB, contributing to delayed or sustained signaling in specific contexts, such as during immune cell differentiation or chronic inflammation.
Recombinant IkBβ protein is engineered for in vitro and in vivo studies to dissect NF-κB pathway dynamics. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains functional domains, including the ankyrin repeats essential for NF-κB interaction and the N-terminal phosphorylation sites that signal proteasomal degradation. Tagged versions (e.g., His-, GST-, or FLAG-tagged) facilitate purification and detection.
Research applications include elucidating IkBβ's role in feedback regulation, its competition with other IkB isoforms, and its impact on gene expression profiles. Recombinant IkBβ is also used to inhibit NF-κB in disease models (e.g., cancer, autoimmune disorders) and to study post-translational modifications (e.g., phosphorylation by IKK complexes). Additionally, structural studies employing recombinant protein have advanced understanding of IkB-NF-κB binding mechanics, aiding therapeutic development targeting NF-κB hyperactivity.
In summary, IkBβ recombinant protein serves as a vital tool for probing NF-κB signaling complexity and exploring interventions in inflammation-related pathologies.
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