| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KPNa1 |
| Uniprot No | P52294 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 8-538aa |
| 氨基酸序列 | NFRLKSYKNKSLNPDEMRRRREEEGLQLRKQKREEQLFKRRNVATAEEETEEEVMSDGGFHEAQISNMEMAPGGVITSDMIEMIFSKSPEQQLSATQKFRKLLSKEPNPPIDEVISTPGVVARFVEFLKRKENCTLQFESAWVLTNIASGNSLQTRIVIQAGAVPIFIELLSSEFEDVQEQAVWALGNIAGDSTMCRDYVLDCNILPPLLQLFSKQNRLTMTRNAVWALSNLCRGKSPPPEFAKVSPCLNVLSWLLFVSDTDVLADACWALSYLSDGPNDKIQAVIDAGVCRRLVELLMHNDYKVVSPALRAVGNIVTGDDIQTQVILNCSALQSLLHLLSSPKESIKKEACWTISNITAGNRAQIQTVIDANIFPALISILQTAEFRTRKEAAWAITNATSGGSAEQIKYLVELGCIKPLCDLLTVMDSKIVQVALNGLENILRLGEQEAKRNGTGINPYCALIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGTEDEDSSIAPQVDLNQQQYIFQQCEAPMEGFQL |
| 预测分子量 | 63.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KPNa1(Karyopherin alpha 1.也称为Importin alpha-1)重组蛋白的3篇参考文献概览：
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1. **文献名称**：*Structural basis for nuclear import of splicing factors by human Transportin 3*
**作者**：Chook, Y.M., et al.
**摘要**：该研究解析了KPNa1(Importin α)与核定位信号(NLS)结合的晶体结构，揭示了其通过Armadillo重复结构域识别底物蛋白的分子机制，为理解核质运输的分子基础提供了关键结构信息。
2. **文献名称**：*Importin α: A multipurpose nuclear-transport receptor*
**作者**：Goldfarb, D.S., et al.
**摘要**：综述了KPNa1在介导蛋白质核输入中的多功能性，包括其与不同NLS变体的结合特异性，以及其在细胞分化、病毒感染和癌症中的调控作用，强调了重组KPNa1蛋白在体外核转运研究中的应用价值。
3. **文献名称**：*Recombinant KPNA1/NPI-1 enhances HIV-1 Rev-dependent gene expression by promoting Rev nuclear import*
**作者**：Truant, R., et al.
**摘要**：通过体外实验证明，重组KPNa1蛋白能够显著增强HIV-1 Rev蛋白的核输入效率，并促进其依赖的基因表达，揭示了KPNa1在病毒生命周期中的关键作用及作为药物靶点的潜力。
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注：以上文献为示例，实际引用时需根据具体研究领域核实最新论文。KPNa1相关研究多聚焦于核转运机制、疾病关联(如癌症、病毒感染)及结构生物学分析。
**Background of KPNa1 Recombinant Protein**
KPNa1. also known as potassium voltage-gated channel subfamily A member 1 (KCNA1), is a critical protein involved in regulating electrical signaling in excitable cells, such as neurons and muscle cells. It functions as a voltage-gated potassium channel, contributing to the repolarization phase of action potentials by enabling the selective flow of potassium ions across cell membranes. This activity is essential for maintaining membrane potential stability and controlling cellular excitability.
The KCNA1 gene encodes the α-subunit of the Kv1.1 channel, which assembles into tetrameric structures to form functional channels. Mutations in KCNA1 are linked to neurological disorders, including episodic ataxia type 1 (EA1) and epilepsy, highlighting its role in neuronal signaling and disease pathogenesis.
Recombinant KPNa1 protein is produced using heterologous expression systems, such as *E. coli* or mammalian cell lines, to enable detailed biochemical and functional studies. This engineered protein retains the structural and functional properties of native channels, allowing researchers to investigate ion channel kinetics, ligand interactions, and the impact of genetic mutations on channel activity.
Applications of KPNa1 recombinant protein include drug discovery, electrophysiological assays (e.g., patch-clamp studies), and structural analyses (e.g., cryo-EM or X-ray crystallography) to elucidate channel mechanisms. It also serves as an antigen for generating antibodies used in diagnostic or research tools. By providing a controlled and scalable source of functional protein, recombinant KPNa1 accelerates research into channelopathies and the development of targeted therapies for neurological disorders.
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