| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | TUBe |
| Uniprot No | P22812 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-462aa |
| 氨基酸序列 | MAYGWNGCGMGVQVNGSNGAIGLSSKYSRNTELRRVEDNDIYRLAKILDENSCWRKLMSIIPKGMDVQACSGAGCLNFPAEIKKGFKYTAQDVFQIDEAANRLPPDQSKSQMMIDEWKTSGKLNERPTVGVLLQLLVQAELFSAADFVALDFLNESTPARPVDGPGALISLELLEEEMEVDNEGLSLKYQSSTATLGADAQGSVGLNLDNFEKDIVRRDKSVPQPSGNTPPIAPPRRQQRSTTNSNFATLTGTGTTSTTIPNVPNLTILNPSEQIQEPVLQPRPMNIPDLSILISNSGDLRATVSDNPSNRTSSTDPPNIPRITLLIDNSGDVNSRPNHAPAKASTATTSTASSNNLPMISALNISKGSRETLRPESRSSSSSLSKDDDDDNDGEEDGEEEYPDAFLPNLSNSEQQSSNNDSSLTTVTGTSGDNSFELTNDSSSTSNDDYACNIPDLSELQQ |
| 预测分子量 | 49,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TUBE(Tandem Ubiquitin Binding Entities)重组蛋白的3-4条参考文献及摘要概括：
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1. **文献名称**：*Efficient protection and isolation of ubiquitylated proteins using tandem ubiquitin-binding entities*
**作者**：Hjerpe, R., et al.
**摘要**：该研究提出利用TUBE重组蛋白高效富集泛素化修饰的靶标蛋白，其多聚泛素结合结构域可特异性识别泛素链，并抑制去泛素化酶(DUBs)的活性，显著提升泛素化蛋白的稳定性和检测灵敏度。
2. **文献名称**：*Structural basis for specific recognition of K63-linked polyubiquitin chains by tandem ubiquitin-binding domains*
**作者**：Sims, J.J., et al.
**摘要**：通过X射线晶体学解析TUBE蛋白与K63型多聚泛素链的结合机制，揭示了其通过串联泛素结合域(UBDs)选择性识别特定泛素链类型的结构基础，为设计泛素化研究工具提供理论支持。
3. **文献名称**：*TUBEs as a tool for studying the ubiquitin-proteasome system in neurodegenerative diseases*
**作者**：López-Sánchez, U., et al.
**摘要**：探讨TUBE重组蛋白在神经退行性疾病(如阿尔茨海默病)研究中的应用，通过富集泛素化蛋白揭示异常蛋白聚集与泛素-蛋白酶体系统失调的关联。
4. **文献名称**：*Development of a high-throughput assay for monitoring ubiquitin chain hydrolysis by deubiquitinating enzymes*
**作者**：Ritorto, M.S., et al.
**摘要**：基于TUBE重组蛋白开发高通量检测方法，用于实时监测去泛素化酶活性，为筛选DUBs抑制剂及研究泛素信号通路提供技术平台。
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以上文献均聚焦于TUBE重组蛋白在泛素化研究中的功能优化、结构解析及疾病模型应用。如需具体文章链接或补充信息，可进一步提供关键词或研究领域细化检索。
**Background of TUBe Recombinant Proteins**
TUBe recombinant proteins are a class of engineered proteins designed to enhance structural and functional studies in molecular biology. Developed as part of advanced biotechnological strategies, TUBe proteins are typically derived from bacteriophage systems, particularly leveraging the T7 phage’s highly efficient replication and expression machinery. The "TUBe" name reflects its association with *T7-based Universal Bioreagent* systems, optimized for rapid, high-yield protein production in bacterial hosts like *E. coli*.
A key feature of TUBe proteins is their modular design, incorporating affinity tags (e.g., His-tag, Strep-tag) and protease cleavage sites to streamline purification and downstream applications. This design minimizes contamination and improves yield, making TUBe variants popular in structural biology (e.g., X-ray crystallography, cryo-EM) and drug discovery. Their compatibility with cell-free expression systems further supports rapid screening of protein-ligand interactions.
TUBe platforms also address challenges in producing membrane proteins and toxic molecules. By coupling T7 RNA polymerase-regulated expression with tunable promoters, researchers can control protein synthesis timing and levels, reducing cellular stress. This adaptability has expanded their use in studying complex targets like GPCRs, ion channels, and viral envelope proteins.
Recent advancements include fusion tags for fluorescent tracking or immunodetection, enhancing their utility in cellular imaging and diagnostic assays. Additionally, TUBe-based toolkits are increasingly employed in synthetic biology for engineering metabolic pathways or biosensors.
Overall, TUBe recombinant proteins represent a versatile, scalable solution for both academic and industrial research, bridging gaps between protein engineering and functional analysis while maintaining cost-effectiveness and reproducibility.
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