| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VB1 |
| Uniprot No | Q9UNZ5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-99aa |
| 氨基酸序列 | MAQGQRKFQAHKPAKSKTAAAASEKNRGPRKGGRVIAPKKARVVQQQKLKKNLEVGIRKKIEHDVVMKASSSLPKKLALLKAPAKKKGAAAATSSKTPS |
| 预测分子量 | 10,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VB1(维生素B1.硫胺素)重组蛋白研究的参考文献示例(文献为虚构，仅作格式参考)：
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1. **文献名称**: *Heterologous Expression and Functional Characterization of Recombinant Thiamine-Binding Protein in E. coli*
**作者**: Smith, J. et al.
**摘要**: 本研究通过大肠杆菌系统成功表达重组硫胺素结合蛋白(VB1结合蛋白)，验证其与硫胺素的高亲和力结合能力，并探讨其在生物传感器开发中的应用潜力。
2. **文献名称**: *Crystal Structure Analysis of Recombinant VB1-Dependent Enzyme from Arabidopsis*
**作者**: Lee, H. & Zhang, Y.
**摘要**: 解析拟南芥来源的VB1依赖性重组酶的晶体结构，阐明其与硫胺素焦磷酸(TPP)辅因子的相互作用机制，为酶工程改造提供结构基础。
3. **文献名称**: *Development of a VB1-Specific Recombinant Antibody for Nutritional Status Monitoring*
**作者**: Gupta, R. et al.
**摘要**: 利用噬菌体展示技术筛选出高特异性重组抗体，可定量检测人体血清中VB1水平，为临床营养缺乏症的快速诊断提供新工具。
4. **文献名称**: *Recombinant VB1 Fusion Protein Enhances Vaccine Immune Response in Mice*
**作者**: Wang, L. et al.
**摘要**: 构建硫胺素结合蛋白与抗原的融合重组蛋白，证明其作为疫苗佐剂可显著提升小鼠模型中的Th1型免疫应答。
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注：以上文献为示例性内容，实际研究中需根据具体方向检索真实数据库(如PubMed)。
**Background of VB1 Recombinant Protein**
The VB1 recombinant protein, derived from the vaccinia virus B1R kinase, is a key component in studying poxvirus biology and host-pathogen interactions. Vaccinia virus, a member of the *Poxviridae* family, has been extensively studied due to its role as a vaccine against smallpox and as a model for viral replication mechanisms. The B1R kinase, encoded by the *B1R* gene, is a serine/threonine kinase critical for viral DNA replication. It phosphorylates host and viral proteins to create a conducive environment for viral replication, particularly by modulating cell cycle checkpoints and neutralizing host antiviral defenses.
Recombinant VB1 protein is produced using genetic engineering techniques, often expressed in *E. coli* or mammalian cell systems, followed by purification via affinity chromatography. Its production enables detailed biochemical and structural studies, such as elucidating kinase-substrate interactions or screening for antiviral inhibitors. Researchers also utilize VB1 to investigate its role in viral evasion of host immune responses, contributing to broader virology and immunology research. Additionally, VB1's homology with cellular kinases makes it a tool for comparative studies on kinase evolution and function.
Beyond basic research, VB1 recombinant protein has applications in drug discovery, particularly in identifying compounds that block poxvirus replication. Its study holds relevance for developing therapeutics against poxviruses and understanding kinase-driven signaling pathways in infectious diseases. Overall, VB1 serves as a vital resource in virology, bridging molecular insights with translational antiviral strategies.
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