| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAM |
| Uniprot No | Q96NU1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-681aa |
| 氨基酸序列 | MSKGILQVHPPICDCPGCRISSPVNRGRLADKRTVALPAARNLKKERTPSFSASDGDSDGSGPTCGRRPGLKQEDGPHIRIMKRRVHTHWDVNISFREASCSQDGNLPTLISSVHRSRHLVMPEHQSRCEFQRGSLEIGLRPAGDLLGKRLGRSPRISSDCFSEKRARSESPQEALLLPRELGPSMAPEDHYRRLVSALSEASTFEDPQRLYHLGLPSHGEDPPWHDPPHHLPSHDLLRVRQEVAAAALRGPSGLEAHLPSSTAGQRRKQGLAQHREGAAPAAAPSFSERELPQPPPLLSPQNAPHVALGPHLRPPFLGVPSALCQTPGYGFLPPAQAEMFAWQQELLRKQNLARLELPADLLRQKELESARPQLLAPETALRPNDGAEELQRRGALLVLNHGAAPLLALPPQGPPGSGPPTPSRDSARRAPRKGGPGPASARPSESKEMTGARLWAQDGSEDEPPKDSDGEDPETAAVGCRGPTPGQAPAGGAGAEGKGLFPGSTLPLGFPYAVSPYFHTGAVGGLSMDGEEAPAPEDVTKWTVDDVCSFVGGLSGCGEYTRVFREQGIDGETLPLLTEEHLLTNMGLKLGPALKIRAQVARRLGRVFYVASFPVALPLQPPTLRAPERELGTGEQPLSPTTATSPYGGGHALAGQTSPKQENGTLALLPGAPDPSQPLC |
| 预测分子量 | 72,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SAM(自组装多肽或S-腺苷甲硫氨酸相关)重组蛋白的参考文献示例，内容为虚构的概括摘要，仅供格式参考：
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1. **文献名称**：*Self-Assembling Peptide-Based Recombinant SAM Fusion Proteins for Drug Delivery*
**作者**：Chen, L., & Zhang, H.
**摘要**：本研究设计了一种基于SAM结构域的重组蛋白，通过基因工程将其与抗癌药物结合，实现pH响应型自组装纳米颗粒，显著提高了药物靶向性并减少系统性毒性。
2. **文献名称**：*Structural and Functional Analysis of SAM Domain in Recombinant TEL Protein*
**作者**：Smith, J.R., et al.
**摘要**：通过X射线晶体学解析了TEL蛋白中SAM结构域的重组表达与三维结构，揭示了其寡聚化机制及在白血病相关信号通路中的调控作用。
3. **文献名称**：*SAM-Dependent Methyltransferase Activity of Recombinant SETD1A Protein*
**作者**：Wang, Y., et al.
**摘要**：利用大肠杆菌系统表达重组SETD1A甲基转移酶，证实其依赖SAM(S-腺苷甲硫氨酸)的组蛋白H3K4甲基化功能，为表观遗传药物开发提供基础。
4. **文献名称**：*Engineering SAM-Regulated Recombinant Proteins for Biosensing Applications*
**作者**：Kim, S., & Patel, R.
**摘要**：开发了一种基于SAM浓度调控的重组荧光蛋白传感器，可实时监测细胞内代谢状态，应用于代谢疾病模型的高通量筛选。
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注：以上文献为示例，实际引用需查询真实数据库(如PubMed、Web of Science)。若需具体领域文献，建议补充关键词(如“SAM结构域”“S-腺苷甲硫氨酸酶”等)。
**Background of SAM Recombinant Proteins**
SAM (Sterile Alpha Motif) recombinant proteins are engineered proteins derived from the SAM domain, a conserved structural motif involved in diverse cellular processes, including protein-protein interactions, signal transduction, and transcriptional regulation. The SAM domain, typically comprising 70 amino acids, forms a helix-loop-helix structure that enables homo- or heterotypic interactions, facilitating the assembly of multi-protein complexes. Originally identified in yeast, SAM domains are now recognized in a wide range of eukaryotes, playing roles in developmental signaling, chromatin remodeling, and apoptosis.
Recombinant SAM proteins are synthesized using recombinant DNA technology, enabling precise control over their expression and purification. By cloning SAM-encoding sequences into expression vectors (e.g., bacterial, insect, or mammalian systems), researchers produce these proteins in vitro for functional and structural studies. This approach allows customization, such as tagging for detection or affinity purification, enhancing their utility in experimental workflows.
SAM proteins are pivotal in studying pathways like the Eph receptor signaling, Polycomb group-mediated gene silencing, and tumor suppressor networks. For instance, SAM-dependent oligomerization of Eph receptors regulates cell-cell communication, while SAM-containing Polycomb proteins govern epigenetic silencing. Dysregulation of SAM-mediated interactions is linked to cancers and neurological disorders, making these proteins valuable targets for therapeutic development.
In research, SAM recombinant proteins are employed in X-ray crystallography, NMR spectroscopy, and biophysical assays to map interaction interfaces. They also serve as tools in pull-down assays, yeast two-hybrid systems, or drug screening platforms to identify inhibitors of pathogenic SAM interactions.
Overall, SAM recombinant proteins bridge structural biology and disease mechanisms, offering insights into modular domain functions and accelerating biomedical applications.
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