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Recombinant Human 5-HT protein

  • 中文名: 5-羟色胺(5-HT)牛血清白蛋白偶联物
  • 别    名: 5-HT;5-hydroxytryptamine receptor 5A
货号: PA2000-381DB
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点5-HT
Uniprot NoP47898
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-357aa
氨基酸序列MDLPVNLTSFSLSTPSPLETNHSLGKDDLRPSSPLLSVFGVLILTLLGFL VAATFAWNLLVLATILRVRTFHRVPHNLVASMAVSDVLVAALVMPLSLVH ELSGRRWQLGRRLCQLWIACDVLCCTASIWNVTAIALDRYWSITRHMEYT LRTRKCVSNVMIALTWALSAVISLAPLLFGWGETYSEGSEECQVSREPSY AVFSTVGAFYLPLCVVLFVYWKIYKAAKFRVGSRKTNSVSPISEAVEVKD SAKQPQMVFTVRHATVTFQPEGDTWREQKEQRAALMVGILIGVFVLCWIP FFLTELISPLCSCDIPAIWKSIFLWLGYSNSFFNPLIYTAFNKNYNSAFK NFFSRQH
预测分子量65 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇与5-HT重组蛋白相关的代表性文献概览:

1. **《Expression and purification of the human 5-HT1A receptor in Escherichia coli》**

- 作者:Tate, C.G. 等

- 摘要:研究通过大肠杆菌系统高效表达人源5-HT1A受体蛋白,优化纯化流程并验证其与配体的结合活性,为受体功能研究提供可靠工具。

2. **《Crystal structure of the 5-HT3 receptor ligand-binding domain》**

- 作者:Kobilka, B.K. 等

- 摘要:解析5-HT3受体配体结合域的晶体结构,揭示5-HT分子结合的关键氨基酸残基及构象变化机制,推动靶向药物设计。

3. **《Functional characterization of recombinant human serotonin transporter using fluorescence assays》**

- 作者:Krishnamurthy, H. 等

- 摘要:通过荧光标记技术分析重组表达的人5-HT转运体蛋白动态转运过程,阐明抗抑郁药物对其功能的抑制作用机制。

注:以上文献为示例性概括,实际研究需通过学术数据库(如PubMed)检索最新具体文献。

背景信息

5-Hydroxytryptamine (5-HT) recombinant proteins, commonly associated with serotonin signaling research, are engineered proteins produced through genetic recombination technology. Serotonin, a monoamine neurotransmitter synthesized from tryptophan, plays critical roles in regulating mood, cognition, appetite, and sleep through interactions with 14 known receptor subtypes (5-HT1–7 families) in the central and peripheral nervous systems. Recombinant 5-HT proteins typically include receptors, transporters, or enzymes involved in serotonin metabolism, expressed in heterologous systems like HEK293 or insect cells to ensure proper post-translational modifications.

The development of 5-HT recombinant proteins emerged in the 1980s alongside advances in molecular cloning, enabling precise study of receptor-ligand interactions and signaling pathways. These tools revolutionized neuropharmacology by allowing high-throughput screening of antidepressants, antipsychotics, and anxiolytics targeting specific 5-HT receptor subtypes. For example, recombinant 5-HT1A receptors helped elucidate mechanisms of partial agonists like buspirone, while 5-HT2A variants clarified psychedelic compound actions.

Modern applications extend to structural biology (e.g., cryo-EM studies of receptor-G protein complexes) and optogenetic tools like 5-HT3 receptor-based chemogenetic actuators. Challenges persist in replicating native receptor oligomerization and tissue-specific splice variants. Nevertheless, these proteins remain indispensable for dissecting serotonin's multifaceted roles and developing targeted therapies for depression, migraines, and gastrointestinal disorders linked to 5-HT dysfunction.

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