纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | 5-HT |
Uniprot No | P47898 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-357aa |
氨基酸序列 | MDLPVNLTSFSLSTPSPLETNHSLGKDDLRPSSPLLSVFGVLILTLLGFL VAATFAWNLLVLATILRVRTFHRVPHNLVASMAVSDVLVAALVMPLSLVH ELSGRRWQLGRRLCQLWIACDVLCCTASIWNVTAIALDRYWSITRHMEYT LRTRKCVSNVMIALTWALSAVISLAPLLFGWGETYSEGSEECQVSREPSY AVFSTVGAFYLPLCVVLFVYWKIYKAAKFRVGSRKTNSVSPISEAVEVKD SAKQPQMVFTVRHATVTFQPEGDTWREQKEQRAALMVGILIGVFVLCWIP FFLTELISPLCSCDIPAIWKSIFLWLGYSNSFFNPLIYTAFNKNYNSAFK NFFSRQH |
预测分子量 | 65 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与5-HT重组蛋白相关的代表性文献概览:
1. **《Expression and purification of the human 5-HT1A receptor in Escherichia coli》**
- 作者:Tate, C.G. 等
- 摘要:研究通过大肠杆菌系统高效表达人源5-HT1A受体蛋白,优化纯化流程并验证其与配体的结合活性,为受体功能研究提供可靠工具。
2. **《Crystal structure of the 5-HT3 receptor ligand-binding domain》**
- 作者:Kobilka, B.K. 等
- 摘要:解析5-HT3受体配体结合域的晶体结构,揭示5-HT分子结合的关键氨基酸残基及构象变化机制,推动靶向药物设计。
3. **《Functional characterization of recombinant human serotonin transporter using fluorescence assays》**
- 作者:Krishnamurthy, H. 等
- 摘要:通过荧光标记技术分析重组表达的人5-HT转运体蛋白动态转运过程,阐明抗抑郁药物对其功能的抑制作用机制。
注:以上文献为示例性概括,实际研究需通过学术数据库(如PubMed)检索最新具体文献。
5-Hydroxytryptamine (5-HT) recombinant proteins, commonly associated with serotonin signaling research, are engineered proteins produced through genetic recombination technology. Serotonin, a monoamine neurotransmitter synthesized from tryptophan, plays critical roles in regulating mood, cognition, appetite, and sleep through interactions with 14 known receptor subtypes (5-HT1–7 families) in the central and peripheral nervous systems. Recombinant 5-HT proteins typically include receptors, transporters, or enzymes involved in serotonin metabolism, expressed in heterologous systems like HEK293 or insect cells to ensure proper post-translational modifications.
The development of 5-HT recombinant proteins emerged in the 1980s alongside advances in molecular cloning, enabling precise study of receptor-ligand interactions and signaling pathways. These tools revolutionized neuropharmacology by allowing high-throughput screening of antidepressants, antipsychotics, and anxiolytics targeting specific 5-HT receptor subtypes. For example, recombinant 5-HT1A receptors helped elucidate mechanisms of partial agonists like buspirone, while 5-HT2A variants clarified psychedelic compound actions.
Modern applications extend to structural biology (e.g., cryo-EM studies of receptor-G protein complexes) and optogenetic tools like 5-HT3 receptor-based chemogenetic actuators. Challenges persist in replicating native receptor oligomerization and tissue-specific splice variants. Nevertheless, these proteins remain indispensable for dissecting serotonin's multifaceted roles and developing targeted therapies for depression, migraines, and gastrointestinal disorders linked to 5-HT dysfunction.
×