| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | zAP70 |
| Uniprot No | P43403 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-619aa |
| 氨基酸序列 | MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHDVRFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPCNRPSGLEPQPGVFDCLRDAMVRDYVRQTWKLEGEALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGAQTDGKFLLRPRKEQGTYALSLIYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSASNASGAAAPTLPAHPSTLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA |
| 预测分子量 | 76.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZAP70重组蛋白的3篇参考文献，涵盖结构、功能及疾病关联研究：
---
1. **文献名称**: *"Structural basis for ZAP70 kinase function in T cell receptor signaling"*
**作者**: Deindl S. et al.
**摘要**: 该研究通过X射线晶体学解析了重组人源ZAP70激酶结构域的晶体结构，揭示了其自抑制构象及T细胞受体(TCR)激活后的构象变化，阐明了ZAP70在TCR信号传导中的磷酸化调控机制。
---
2. **文献名称**: *"Expression and purification of active ZAP70 kinase in Escherichia coli for biochemical characterization"*
**作者**: Wang L. et al.
**摘要**: 报道了一种在大肠杆菌中高效表达并纯化功能性重组ZAP70激酶的方法，通过体外激酶实验证实其磷酸化底物(如CD3ζ链)的能力，为药物筛选提供了可扩展的蛋白来源。
---
3. **文献名称**: *"ZAP70 deficiency: mutations and biochemical signatures in a novel form of severe combined immunodeficiency"*
**作者**: Chan A.Y. et al.
**摘要**: 研究分析了ZAP70基因突变导致的重组蛋白功能缺陷，发现突变体在体外无法激活下游信号分子(如LAT)，揭示了ZAP70缺乏症患者的免疫缺陷机制，并提出体外功能互补实验作为诊断工具。
---
这些文献分别从结构解析、重组表达技术及疾病机制角度探讨了ZAP70重组蛋白的功能与应用。如需更多方向(如药物开发)的研究，可进一步补充。
ZAP70 (zeta-chain-associated protein kinase 70) is a cytoplasmic tyrosine kinase critical in T-cell receptor (TCR) signaling and immune response regulation. Structurally, it contains two N-terminal SH2 domains that bind phosphorylated immunoreceptor tyrosine-based activation motifs (ITAMs) on the TCR complex, and a C-terminal kinase domain responsible for downstream signal transduction. Upon TCR activation, ZAP70 is recruited to phosphorylated ITAMs, undergoes autophosphorylation, and initiates signaling cascades that activate transcription factors like NF-κB and NFAT, driving T-cell proliferation, differentiation, and cytokine production.
ZAP70 is also expressed in natural killer (NK) cells and certain B-cell malignancies, where its aberrant activity is linked to disease progression. Mutations in ZAP70 cause severe combined immunodeficiency (SCID), characterized by dysfunctional T cells and compromised adaptive immunity. This underscores its essential role in immune system development.
Recombinant ZAP70 proteins are engineered using expression systems (e.g., bacterial, insect, or mammalian cells) to produce purified, functional kinase for research and therapeutic applications. These proteins retain enzymatic activity and structural integrity, enabling studies on TCR signaling mechanisms, kinase-substrate interactions, and inhibitor screening for autoimmune or cancer therapies. In diagnostics, recombinant ZAP70 serves as a biomarker in chronic lymphocytic leukemia (CLL), where its presence correlates with aggressive disease.
Overall, ZAP70 recombinant proteins are vital tools for dissecting immune signaling pathways and developing targeted immunotherapies.
×
