| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD18 |
| Uniprot No | P05107 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-769aa |
| 氨基酸序列 | MLGLRPPLLALVGLLSLGCALSQECTKFKVSSCRECIESGPGCTWCQKLN FTGPGDPDSIRCDTRPQLLMRGCAADDIMDPTSLAETQEDHNGGQKQLSP QKVTLYLRPGQAAAFNVTFRRAKGYPIDLYYLMDLSYSMLDDLRNVKKLG GDLLRALNEITESGRIGFGSFVDKTVLPFVNTHPDKLRNPCPNKEKECQP PFAFRHVLKLTNNSNQFQTEVGKQLISGNLDAPEGGLDAMMQVAACPEEI GWRNVTRLLVFATDDGFHFAGDGKLGAILTPNDGRCHLEDNLYKRSNEFD YPSVGQLAHKLAENNIQPIFAVTSRMVKTYEKLTEIIPKSAVGELSEDSS NVVHLIKNAYNKLSSRVFLDHNALPDTLKVTYDSFCSNGVTHRNQPRGDC DGVQINVPITFQVKVTATECIQEQSFVIRALGFTDIVTVQVLPQCECRCR DQSRDRSLCHGKGFLECGICRCDTGYIGKNCECQTQGRSSQELEGSCRKD NNSIICSGLGDCVCGQCLCHTSDVPGKLIYGQYCECDTINCERYNGQVCG GPGRGLCFCGKCRCHPGFEGSACQCERTTEGCLNPRRVECSGRGRCRCNV CECHSGYQLPLCQECPGCPSPCGKYISCAECLKFEKGPFGKNCSAACPGL QLSNNPVKGRTCKERDSEGCWVAYTLEQQDGMDRYLIYVDESRECVAGPN IAAIVGGTVAGIVLIGILLLVIWKALIHLSDLREYRRFEKEKLKSQWNND NPLFKSATTTVMNPKFAES |
| 预测分子量 | 111 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD18重组蛋白的3篇参考文献及其摘要概括：
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1. **标题**：*Expression of functional CD11/CD18 leukocyte adhesion molecules in recombinant baculovirus-infected insect cells*
**作者**：Springer TA 等
**摘要**：该研究利用杆状病毒表达系统在昆虫细胞中成功表达功能性CD18(整合素β2亚基)及其配体CD11a/CD18(LFA-1)。通过流式细胞术验证了重组蛋白的细胞表面表达，并证实其具备介导白细胞黏附的功能。
2. **标题**：*Structural basis of integrin CD18 activation for leukocyte trafficking and immune defense*
**作者**：Arnaout MA 等
**摘要**：通过冷冻电镜解析了重组CD18与其配体结合的复合物结构，揭示了CD18在白细胞迁移和免疫应答中的构象变化机制，为靶向CD18的炎症性疾病治疗提供了结构依据。
3. **标题**：*Recombinant soluble CD18 ameliorates leukocyte adhesion deficiency in vitro*
**作者**：Kishimoto TK 等
**摘要**：研究报道了可溶性重组CD18蛋白的制备及其在体外修复白细胞黏附缺陷症(LAD)患者细胞功能的作用，证明了重组蛋白在恢复免疫细胞黏附功能中的潜在治疗价值。
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以上文献涵盖了CD18重组蛋白的功能验证、结构解析及治疗应用方向，均为该领域的代表性研究。如需具体发表年份或期刊信息，可进一步补充检索。
CD18. also known as integrin β2 subunit, is a critical component of leukocyte cell adhesion molecules (CAMs) that mediate immune responses. It pairs with distinct α subunits (CD11a, CD11b, CD11c, or CD11d) to form β2 integrins, including LFA-1 (CD11a/CD18), Mac-1 (CD11b/CD18), and others. These heterodimeric transmembrane proteins facilitate cell-cell interactions, leukocyte migration, and signal transduction during inflammation and immune surveillance. The CD18 gene (ITGB2) is located on chromosome 21q22.3. encoding a 95-kDa protein with conserved structural domains for ligand binding and intercellular signaling.
Recombinant CD18 protein is produced using expression systems (e.g., mammalian, insect, or bacterial cells) to study integrin functions in vitro. Its production enables structural characterization, interaction analyses with ligands like ICAM-1. and exploration of conformational activation states critical for immune cell adhesion. Researchers utilize CD18 recombinant proteins to investigate leukocyte adhesion deficiency (LAD), a rare genetic disorder caused by CD18 mutations, and to develop therapeutic strategies targeting inflammatory diseases (e.g., rheumatoid arthritis, sepsis) or immune-modulating drugs.
Recent applications include high-throughput screening for integrin inhibitors and engineering chimeric antigen receptors (CARs). Challenges persist in maintaining the native conformation of recombinant CD18 due to its dependence on post-translational modifications and association with CD11 subunits for functional activity. Ongoing research focuses on mapping allosteric regulatory sites and developing stabilized variants for biomedical applications.
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