| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CENTa2 |
| Uniprot No | Q9NPF8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-381aa |
| 氨基酸序列 | MGDRERNKKR LLELLRAPDT GNAHCADCGA ADPDWASYKL GIFICLNCCG VHRNFPDISR VKSVRLDFWD DSIVEFMIHN GNLRVKAKFE ARVPAFYYIP QANDCLVLKE QWIRAKYERR EFMADGETIS LPGNREGFLW KRGRDNSQFL RRKFVLLARE GLLKYFTKEQ GKSPKAVISI KDLNATFQTE KIGHPHGLQI TYRRDGHTRN LFVYHESGKE IVDWFNALRA ARLQYLKMAF PELPESELVP FLTRNYLKQG FMEKTGPKQK EPFKKRWFAL DCHERRLLYY KNPLDAFEQG QVFLGNKEQG YEAYEDLPKG IRGNRWKAGL TIVTPERRFV LTCPSEKEQQ EWLESLRGVL SSPLTPLNRL TASTESGRSS R |
| 预测分子量 | 44,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CENTA2重组蛋白的模拟参考文献示例(注：为示例性内容，非真实文献)：
1. **《重组CENTA2蛋白的表达及其磷酸肌醇结合特性分析》**
- 作者：Smith, J. et al.
- 摘要：本研究在大肠杆菌中成功表达并纯化重组CENTA2蛋白，证实其通过PH结构域特异性结合磷酸肌醇(如PIP3)，揭示了其在细胞信号转导中的潜在作用。
2. **《CENTA2调控细胞周期的分子机制研究》**
- 作者：Zhang, L. et al.
- 摘要：通过体外实验发现重组CENTA2蛋白与CDK1激酶相互作用，影响G2/M期转换，表明CENTA2可能通过调控细胞周期蛋白参与肿瘤发生。
3. **《CENTA2蛋白的晶体结构解析及功能域鉴定》**
- 作者：Lee, S. et al.
- 摘要：利用X射线晶体学解析了重组CENTA2蛋白的三维结构，发现其N端的Arf-GAP结构域对调控细胞膜重塑至关重要，为靶向药物设计提供了结构基础。
4. **《基于重组CENTA2的抗体开发及其在乳腺癌诊断中的应用》**
- 作者：Chen, Y. et al.
- 摘要：以重组CENTA2蛋白为抗原制备单克隆抗体，临床样本验证显示其在乳腺癌组织中高表达，提示其作为新型生物标志物的潜力。
如需真实文献，建议通过PubMed或Google Scholar以“CENTA2 recombinant protein”、“CENTA2 expression”等关键词检索，并筛选近年研究。
**Background of CENTa2 Recombinant Protein**
CENTa2 is a synthetic recombinant protein engineered to mimic or enhance specific biological functions derived from natural centaurin-alpha family proteins, particularly centaurin-alpha 1 (CENTa1), which play roles in intracellular signaling and membrane trafficking. Centaurin proteins are characterized by their distinctive structural domains, including pleckstrin homology (PH) domains that bind phosphoinositides, and ArfGAP domains involved in regulating ADP-ribosylation factor (Arf) proteins. These features enable their participation in critical cellular processes such as vesicle formation, cytoskeletal organization, and signal transduction pathways linked to cell growth and survival.
The development of CENTa2 as a recombinant protein arose from the need to study centaurin-alpha functions with higher specificity or to modulate pathways implicated in diseases like cancer, neurodegeneration, or metabolic disorders. Unlike native centaurin-alpha proteins, CENTa2 is often designed with optimized domains or tags (e.g., His-tag, GST-tag) for improved solubility, purification, and detection in experimental settings. Researchers utilize it in *in vitro* assays, structural studies, or cellular models to dissect mechanisms of phosphoinositide signaling or Arf-dependent trafficking.
Additionally, CENTa2 has potential therapeutic applications. By interfering with or enhancing specific interactions, it could regulate aberrant signaling in pathological conditions. Its recombinant form allows scalable production via bacterial or eukaryotic expression systems, ensuring consistency for functional studies. Ongoing research focuses on elucidating its binding partners, downstream effects, and therapeutic feasibility, positioning CENTa2 as a versatile tool for both basic science and drug discovery.
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