| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSPA1B |
| Uniprot No | P0DMV9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-641aa |
| 氨基酸序列 | MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKPKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD |
| 预测分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于HSPA1B重组蛋白研究的模拟参考文献示例(注：文献信息为虚构，实际研究需查阅真实数据库)：
1. **《重组HSPA1B蛋白在大肠杆菌中的高效表达与纯化》**
- 作者：Chen L., Wang Y.
- 摘要：研究通过构建pET载体在大肠杆菌中高效表达HSPA1B重组蛋白，优化诱导条件并采用镍柱亲和层析纯化，获得高纯度蛋白用于后续功能研究。
2. **《HSPA1B对氧化应激诱导的细胞凋亡保护机制研究》**
- 作者：Kim S., Tanaka H.
- 摘要：发现重组HSPA1B通过调控Bcl-2/Bax通路抑制氧化应激导致的线粒体凋亡，证实其在细胞保护中的关键作用。
3. **《HSPA1B重组蛋白在神经炎症模型中的治疗潜力》**
- 作者：Gomez R., et al.
- 摘要：在小鼠神经炎症模型中，外源性HSPA1B重组蛋白显著降低促炎因子TNF-α和IL-6水平，提示其潜在抗炎治疗价值。
4. **《HSPA1B与Tau蛋白相互作用的分子动力学模拟》**
- 作者：Zhang X., Liu F.
- 摘要：利用重组HSPA1B和分子对接技术，揭示其通过特定结构域与病理性Tau蛋白结合，可能延缓阿尔茨海默病进展。
建议通过PubMed或Web of Science检索真实文献，关键词组合如"HSPA1B recombinant protein expression"或"HSPA1B functional analysis"。
**Background of HSPA1B Recombinant Protein**
HSPA1B, a member of the heat shock protein 70 (HSP70) family, is a molecular chaperone critical for cellular stress response and protein homeostasis. It is encoded by the *HSPA1B* gene, located on chromosome 6 in humans, and is highly inducible under stress conditions such as heat, toxins, or hypoxia. HSPA1B assists in folding nascent polypeptides, preventing protein aggregation, and facilitating the translocation of proteins across cellular membranes. It binds to exposed hydrophobic regions of misfolded proteins in an ATP-dependent manner, stabilizing them during refolding or degradation.
Recombinant HSPA1B protein is produced using biotechnological methods, often expressed in *E. coli*, yeast, or mammalian cell systems to ensure proper folding and post-translational modifications. Purification typically involves affinity chromatography (e.g., His-tag systems) followed by validation via SDS-PAGE, Western blot, or mass spectrometry. Functionally active recombinant HSPA1B exhibits ATPase activity and interacts with co-chaperones like HSP40 or BAG-1. which regulate its substrate-binding cycle.
Research on HSPA1B focuses on its roles in diseases, including cancer, neurodegenerative disorders (e.g., Alzheimer’s), and cardiovascular conditions, where cellular stress pathways are dysregulated. It is also studied as a potential biomarker for stress responses or therapeutic target, with efforts to develop HSP70 inhibitors to disrupt cancer cell survival. Additionally, recombinant HSPA1B serves as a tool to study protein-protein interactions, screen drug candidates, or engineer chaperone-based therapies for protein misfolding diseases.
In summary, HSPA1B recombinant protein bridges basic research and translational applications, offering insights into stress biology and avenues for novel diagnostics or therapeutics.
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