| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTSW |
| Uniprot No | P56202 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-376aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSIRGPLRA QDLGPQPLEL KEAFKLFQIQ FNRSYLSPEE HAHRLDIFAH NLAQAQRLQE EDLGTAEFGV TPFSDLTEEE FGQLYGYRRA AGGVPSMGRE IRSEEPEESV PFSCDWRKVA GAISPIKDQK NCNCCWAMAA AGNIETLWRI SFWDFVDVSV QELLDCGRCG DGCHGGFVWD AFITVLNNSG LASEKDYPFQ GKVRAHRCHP KKYQKVAWIQ DFIMLQNNEH RIAQYLATYG PITVTINMKP LQLYRKGVIK ATPTTCDPQL VDHSVLLVGF GSVKSEEGIW AETVSSQSQP QPPHPTPYWI LKNSWGAQWG EKGYFRLHRG SNTCGITKFP LTARVQKPDM KPRVSCPP |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CTSW(组织蛋白酶W)重组蛋白研究的参考文献摘要整理(注：内容基于公开文献归纳，非真实引用，仅作示例参考)：
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1. **文献名称**：*"Recombinant human Cathepsin W (CTSW) expression in HEK293 cells and its role in T-cell cytotoxicity"*
**作者**：Müller et al.
**摘要**：研究通过HEK293细胞系统成功表达并纯化重组人CTSW蛋白，证实其在体外可激活T细胞颗粒酶途径，并揭示其通过调节穿孔素活性参与抗病毒免疫反应的机制。
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2. **文献名称**：*"Structural and functional characterization of CTSW recombinant protein in cancer cell invasion"*
**作者**：Zhang Y, et al.
**摘要**：通过原核表达系统获得高纯度CTSW重组蛋白，结合晶体结构分析发现其底物结合域的关键位点，实验证明CTSW通过降解细胞外基质蛋白促进肿瘤细胞迁移，提示其作为癌症治疗靶点的潜力。
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3. **文献名称**：*"CTSW autoantibodies detected by recombinant protein-based ELISA in autoimmune diseases"*
**作者**：Tanaka K, et al.
**摘要**：利用重组CTSW蛋白建立新型ELISA检测方法，发现类风湿关节炎患者血清中CTSW自身抗体水平显著升高，表明CTSW可能参与自身免疫病理过程，为疾病诊断提供新标志物。
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如需具体文献，建议通过PubMed或Web of Science以关键词“Cathepsin W recombinant protein”检索真实发表论文。
**Background of CTSW Recombinant Protein**
Cathepsin W (CTSW), a member of the cysteine cathepsin protease family, is a lysosomal protease predominantly expressed in cytotoxic lymphocytes, including natural killer (NK) cells and CD8+ T-cells. Unlike other cathepsins, CTSW exhibits restricted tissue distribution, suggesting specialized roles in immune regulation. It is synthesized as an inactive zymogen, requiring proteolytic cleavage for activation. Structurally, CTSW shares conserved features with cysteine proteases, including a propeptide region and a catalytic domain with a cysteine-histidine active site.
Recombinant CTSW protein is produced using biotechnological platforms (e.g., bacterial, insect, or mammalian expression systems) to enable controlled study of its biochemical and functional properties. Its recombinant form often retains enzymatic activity, allowing researchers to investigate substrate specificity, pH-dependent activity, and interactions with endogenous inhibitors like cystatins. CTSW is implicated in immune responses, particularly in cytotoxic lymphocyte-mediated apoptosis and antigen processing. Studies suggest its potential involvement in antiviral defense, tumor surveillance, and autoimmune disorders, though its precise mechanisms remain under exploration.
The development of CTSW recombinant protein has advanced therapeutic research, particularly in immunology and oncology. It serves as a tool for screening protease inhibitors, designing targeted therapies, or studying immune cell cytotoxicity. Additionally, dysregulated CTSW expression has been observed in certain cancers and inflammatory diseases, highlighting its diagnostic or prognostic potential. However, challenges persist in understanding its physiological substrates and regulatory pathways, necessitating further structural and functional studies. Overall, CTSW recombinant protein represents a critical reagent for deciphering its role in immunity and disease pathogenesis.
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