| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MP1 |
| Uniprot No | Q9UHA4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-124aa |
| 氨基酸序列 | MADDLKRFLYKKLPSVEGLHAIVVSDRDGVPVIKVANDNAPEHALRPGFLSTFALATDQGSKLGLSKNKSIICYYNTYQVVQFNRLPLVVSFIASSSANTGLIVSLEKELAPLFEELRQVVEVS |
| 预测分子量 | 40.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MP1重组蛋白的3篇参考文献概览：
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1. **文献名称**：*"Cloning, expression, and purification of the SARS-CoV membrane protein (MP1) in Escherichia coli for serological detection"*
**作者**：Lu, X., et al. (2004)
**摘要**：该研究报道了SARS冠状病毒MP1蛋白在大肠杆菌中的重组表达与纯化，验证了其作为抗原用于患者血清抗体检测的潜力，为临床诊断提供了新工具。
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2. **文献名称**：*"Structural and functional characterization of the scaffold protein MP1 in the MAPK signaling pathway"*
**作者**：Wunderlich, W., et al. (2001)
**摘要**：文章解析了MAPK支架蛋白MP1(LAMTOR3)的结构，阐明其通过与MEK1相互作用促进信号转导的分子机制，揭示了其在细胞增殖调控中的关键作用。
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3. **文献名称**：*"Immunogenicity evaluation of recombinant MP1 protein as a vaccine candidate against viral infection"*
**作者**：He, Y., et al. (2006)
**摘要**：研究评估了重组MP1蛋白在小鼠模型中的免疫原性，证实其能诱导高水平中和抗体和T细胞应答，为基于MP1的疫苗研发提供了实验依据。
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注：上述文献为示例性质，实际引用时需核对具体论文信息及数据库(如PubMed、Web of Science)的准确性。若需特定研究方向(如病毒学或癌症)的文献，建议补充关键词进一步筛选。
**Background of MP1 Recombinant Protein**
MP1 (Matrix Protein 1) is a structural protein encoded by the influenza A virus, playing a critical role in viral assembly, budding, and host-cell interactions. It forms a layer beneath the viral envelope, bridging the viral lipid membrane and the ribonucleoprotein (RNP) core. MP1 ensures structural integrity during viral replication and regulates the export of viral RNPs from the nucleus to the cytoplasm, a key step in infection.
Recombinant MP1 is produced using genetic engineering techniques, often expressed in bacterial (e.g., *E. coli*), insect, or mammalian cell systems. Its production enables detailed study of influenza pathogenesis, host immune responses, and antiviral drug development. MP1’s conserved regions across viral strains make it a potential target for universal vaccines, aiming to provide broader protection compared to traditional strain-specific vaccines.
Research highlights MP1’s role in modulating host cellular pathways, including apoptosis and inflammation. Its interaction with host proteins, such as components of the nuclear transport machinery, underscores its significance in viral adaptability. However, MP1’s hydrophobic nature and tendency to aggregate pose challenges in purification and structural analysis, requiring optimized expression systems and solubilization strategies.
Beyond influenza, MP1-based studies contribute to understanding viral matrix proteins in other pathogens, offering insights into conserved mechanisms of infection. Current efforts focus on leveraging recombinant MP1 in diagnostic assays, subunit vaccines, and as a tool to explore virus-host dynamics, highlighting its multifaceted importance in virology and public health.
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