| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAMC3 |
| Uniprot No | Q9Y6N6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1575aa |
| 氨基酸序列 | MAAAALLLGLALLAPRAAGAGMGACYDGAGRPQRCLPVFENAAFGRLAQASHTCGSPPEDFCPHVGAAGAGAHCQRCDAADPQRHHNASYLTDFHSQDESTWWQSPSMAFGVQYPTSVNITLRLGKAYEITYVRLKFHTSRPESFAIYKRSRADGPWEPYQFYSASCQKTYGRPEGQYLRPGEDERVAFCTSEFSDISPLSGGNVAFSTLEGRPSAYNFEESPGLQEWVTSTELLISLDRLNTFGDDIFKDPKVLQSYYYAVSDFSVGGRCKCNGHASECGPDVAGQLACRCQHNTTGTDCERCLPFFQDRPWARGTAEAAHECLPCNCSGRSEECTFDRELFRSTGHGGRCHHCRDHTAGPHCERCQENFYHWDPRMPCQPCDCQSAGSLHLQCDDTGTCACKPTVTGWKCDRCLPGFHSLSEGGCRPCTCNPAGSLDTCDPRSGRCPCKENVEGNLCDRCRPGTFNLQPHNPAGCSSCFCYGHSKVCASTAQFQVHHILSDFHQGAEGWWARSVGGSEHPPQWSPNGVLLSPEDEEELTAPEKFLGDQRFSYGQPLILTFRVPPGDSPLPVQLRLEGTGLALSLRHSSLSGPQDAGHPREVELRFHLQETSEDVAPPLPPFHFQRLLANLTSLRLRVSPGPSPAGPVFLTEVRLTSARPGLSPPASWVEICSCPTGYTGQFCESCAPGYKREMPQGGPYASCVPCTCNQHGTCDPNTGICVCSHHTEGPSCERCLPGFYGNPFAGQADDCQPCPCPGQSACTTIPESREVVCTHCPPGQRGRRCEVCDDGFFGDPLGLFGHPQPCHQCQCSGNVDPNAVGNCDPLSGHCLRCLHNTTGDHCEHCQEGFYGSALAPRPADKCMPCSCHPQGSVSEQMPCDPVTGQCSCLPHVTARDCSRCYPGFFDLQPGRGCRSCKCHPLGSQEDQCHPKTGQCTCRPGVTGQACDRCQLGFFGFSIKGCRACRCSPLGAASAQCHENGTCVCRPGFEGYKCDRCHDNFFLTADGTHCQQCPSCYALVKEEAAKLKARLTLTEGWLQGSDCGSPWGPLDILLGEAPRGDVYQGHHLLPGAREAFLEQMMSLEGAVKAAREQLQRLNKGARCAQAGSQKTCTQLADLEAVLESSEEEILHAAAILASLEIPQEGPSQPTKWSHLATEARALARSHRDTATKIAATAWRALLASNTSYALLWNLLEGRVALETQRDLEDRYQEVQAAQKALRTAVAEVLPEAESVLATVQQVGADTAPYLALLASPGALPQKSRAEDLGLKAKALEKTVASWQHMATEAARTLQTAAQATLRQTEPLTKLHQEARAALTQASSSVQAATVTVMGARTLLADLEGMKLQFPRPKDQAALQRKADSVSDRLLADTRKKTKQAERMLGNAAPLSSSAKKKGREAEVLAKDSAKLAKALLRERKQAHRRASRLTSQTQATLQQASQQVLASEARRQELEEAERVGAGLSEMEQQIRESRISLEKDIETLSELLARLGSLDTHQAPAQALNETQWALERLRLQLGSPGSLQRKLSLLEQESQQQELQIQGFESDLAEIRADKQNLEAILHSLPENCASWQ |
| 预测分子量 | 171 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LAMC3重组蛋白的参考文献示例(注：以下为模拟文献，实际文献需通过学术数据库检索获取)：
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1. **文献名称**: *Recombinant LAMC3 promotes tumor cell invasion through integrin-mediated signaling pathways*
**作者**: Yilmaz, A., et al. (2019)
**摘要**: 该研究通过在大肠杆菌中表达并纯化重组人LAMC3蛋白，探究其在肿瘤微环境中的作用。实验表明，重组LAMC3通过激活整合素-β1信号通路，显著增强肺癌细胞的迁移和侵袭能力。
2. **文献名称**: *Structural and functional characterization of the laminin γ3 chain using recombinant protein technology*
**作者**: Smith, J.P., et al. (2017)
**摘要**: 作者利用哺乳动物表达系统制备了功能性重组LAMC3蛋白，并通过X射线晶体学解析其三维结构。研究发现，LAMC3的C端结构域在介导细胞黏附中起关键作用，为开发靶向层粘连蛋白的疗法提供依据。
3. **文献名称**: *LAMC3 recombinant protein rescues neuronal defects in a zebrafish knockout model*
**作者**: Chen, L., et al. (2020)
**摘要**: 研究通过体外表达重组LAMC3蛋白，验证其在斑马鱼神经发育中的功能。实验表明，外源性LAMC3可部分恢复LAMC3基因敲除模型的突触形成异常，提示其在神经系统中具有调控轴突导向的作用。
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**建议**：如需真实文献，可使用以下关键词在PubMed或Google Scholar检索：
- "LAMC3 recombinant protein expression"
- "LAMC3 functional analysis"
- "Laminin gamma 3 recombinant"
LAMC3. a member of the laminin γ-chain family, is a critical component of laminin heterotrimers—extracellular matrix proteins essential for tissue organization and cellular communication. As one of the three chains (α, β, γ) forming laminin isoforms, the γ3 chain encoded by LAMC3 contributes to the structural and functional diversity of laminins. Laminins play pivotal roles in cell adhesion, differentiation, and tissue development by interacting with cell surface receptors like integrins and dystroglycans.
Recombinant LAMC3 protein is engineered using expression systems (e.g., mammalian cells, bacteria) to produce purified γ3 chains for research and therapeutic applications. Its production enables detailed studies of laminin assembly, receptor binding, and signaling pathways in processes such as epithelial formation, neuronal development, and wound healing. Notably, LAMC3-containing laminins are implicated in cancer progression and metastasis, making the recombinant protein valuable for oncology research.
The development of recombinant LAMC3 addresses challenges in studying native laminins, which are difficult to isolate due to complex heterotrimerization and low abundance. By providing a controlled, scalable source, it facilitates mechanistic investigations and preclinical testing of laminin-targeted therapies. Current research focuses on optimizing its bioactivity, stability, and compatibility with biomaterials for tissue engineering. Challenges remain in replicating post-translational modifications critical for its native function, driving advances in expression technologies. As a tool, recombinant LAMC3 enhances our understanding of extracellular matrix biology and its translational potential in regenerative medicine.
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