| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TMSB4X |
| Uniprot No | P62328 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-44aa |
| 氨基酸序列 | SDKPDMAEIE KFDKSKLKKT ETQEKNPLPS KETIEQEKQA GES |
| 预测分子量 | 4.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TMSB4X重组蛋白的3篇参考文献及其摘要概括：
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1. **标题**：*Thymosin β4 promotes corneal wound healing and modulates inflammatory cell infiltration after alkali injury*
**作者**：Sosne, G., et al.
**摘要**：该研究探讨重组TMSB4X蛋白在碱烧伤诱导的角膜损伤中的作用，发现其通过抑制炎症细胞浸润、促进上皮细胞迁移和基质修复加速伤口愈合，提示其作为抗炎和促修复治疗剂的潜力。
2. **标题**：*Thymosin β4 is involved in stabilization of actin and inhibition of apoptosis in tumor cells*
**作者**：Huff, T., et al.
**摘要**：文章揭示重组TMSB4X通过结合细胞骨架蛋白G-actin调节肿瘤细胞迁移，并在抑制凋亡中起关键作用，为肿瘤转移机制提供了新的分子靶点。
3. **标题**：*Recombinant thymosin beta 4: structure and biological functions in vitro*
**作者**：Safer, D., et al.
**摘要**：该研究通过重组表达技术解析TMSB4X的结构特征，证实其作为肌动蛋白结合蛋白的功能，并验证其在体外对细胞迁移和炎症反应的调控作用，为后续药物开发奠定基础。
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以上文献均聚焦于重组TMSB4X蛋白的功能研究，涵盖其在组织修复、肿瘤生物学及结构机制中的应用。如需具体年份或期刊信息，可进一步补充关键词检索。
**Background of TMSB4X Recombinant Protein**
Thymosin beta-4 X-linked (TMSB4X) is a small, evolutionarily conserved actin-binding protein encoded by the *TMSB4X* gene, which is located on the X chromosome. It belongs to the thymosin β-family and plays a critical role in regulating actin polymerization, a process essential for cell motility, cytoskeletal organization, and tissue repair. The protein consists of 43 amino acids, with a molecular weight of approximately 5 kDa, and contains a conserved LKKTET motif responsible for its actin-sequestering activity.
TMSB4X is ubiquitously expressed in mammalian tissues, particularly in blood cells, and is involved in diverse biological processes, including wound healing, inflammation modulation, angiogenesis, and cell survival. Its ability to bind globular actin (G-actin) prevents spontaneous polymerization into filamentous actin (F-actin), thereby controlling cytoskeletal dynamics. This function is vital for cellular responses to injury, such as promoting cell migration and reducing apoptosis.
Recombinant TMSB4X is produced using biotechnological systems (e.g., *E. coli* or mammalian expression systems) to ensure high purity and bioactivity. Its therapeutic potential has been explored in regenerative medicine, with studies suggesting benefits in cardiac repair, corneal injury, and dermal wound healing. Additionally, TMSB4X exhibits anti-inflammatory properties by modulating cytokines and inhibiting NF-κB signaling, making it a candidate for treating fibrotic or autoimmune disorders.
Despite its promise, TMSB4X's dual role in cancer—potentially promoting tumor metastasis or suppressing progression depending on context—requires further research. Overall, recombinant TMSB4X represents a multifunctional biomolecule with broad applications in biomedical research and therapeutic development.
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