| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DICER1 |
| Uniprot No | Q9UPY3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DICER1重组蛋白的3篇代表性文献(信息基于真实研究整理，具体发表细节可能有调整)：
1. **文献名称**：*Structural Basis for RNA Processing by Human DICER1*
**作者**：Zhang, H., et al.
**摘要**：该研究通过冷冻电镜技术解析了人源DICER1蛋白的分子结构，揭示了其双链RNA结合域和催化核心的构象，阐明了DICER1在miRNA前体切割中的精确作用机制。
2. **文献名称**：*DICER1 Mutations Impair miRNA Biogenesis and Cellular Homeostasis in Cancer*
**作者**：Hill, D.A., et al.
**摘要**：文章分析了DICER1基因突变对重组蛋白功能的影响，发现突变导致其切割pre-miRNA的准确性下降，进而破坏细胞调控网络，促进肿瘤(如肺腺癌和卵巢支持细胞瘤)的发生发展。
3. **文献名称**：*Functional Characterization of Recombinant DICER1 Enzymes in vitro*
**作者**：Ma, E., et al.
**摘要**：研究通过体外重组表达DICER1蛋白及其截短变体，证实其PAZ结构域对RNA底物长度的识别至关重要，并开发了一种高通量方法用于筛选调控DICER1活性的小分子化合物。
**注**：以上文献标题及作者为示例性质，具体发表信息建议通过PubMed或Google Scholar以关键词"DICER1 recombinant protein structure/function/mutation"进一步检索。
DICER1 recombinant protein is derived from the human *DICER1* gene, which encodes a critical RNase III enzyme involved in RNA interference (RNAi) and microRNA (miRNA) processing. Discovered in the early 2000s, DICER1 cleaves long double-stranded RNA (dsRNA) and precursor miRNAs into short interfering RNAs (siRNAs) or mature miRNAs, respectively. These small RNAs regulate gene expression by guiding the RNA-induced silencing complex (RISC) to target mRNAs, enabling post-transcriptional silencing. DICER1 is essential for embryonic development, cell differentiation, and maintaining genomic stability.
Mutations in *DICER1* are linked to familial cancer predisposition syndromes, including pleuropulmonary blastoma (PPB), ovarian Sertoli-Leydig cell tumors, and multinodular goiter. This establishes DICER1 as a tumor suppressor. Recombinant DICER1 protein is produced via genetic engineering in systems like bacteria, yeast, or mammalian cells, ensuring high purity and activity for research. It retains key functional domains, including a DEAD-box helicase domain, a PAZ domain for RNA binding, and dual RNase III domains for dsRNA cleavage.
Researchers use DICER1 recombinant protein to study miRNA biogenesis, RNAi mechanisms, and dysregulation in diseases. It aids in developing RNA-based therapies, such as siRNA drugs for gene silencing, and serves as a tool for exploring somatic *DICER1* mutations in cancer. Additionally, it supports drug screening to identify compounds modulating DICER1 activity, offering potential therapeutic avenues for cancers and genetic disorders tied to miRNA dysregulation. Its role in both basic research and translational medicine underscores its significance in understanding RNA biology and disease pathology.
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