| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARPC4 |
| Uniprot No | P59998 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-168aa |
| 氨基酸序列 | TATLRPYLS AVRATLQAAL CLENFSSQVV ERHNKPEVEV RSSKELLLQP VTISRNEKEK VLIEGSINSV RVSIAVKQAD EIEKILCHKF MRFMMMRAEN FFILRRKPVE GYDISFLITN FHTEQMYKHK LVDFVIHFME EIDKEISEMK LSVNARARIV AEEFLKNF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **ARPC4重组蛋白** 的参考文献示例(注：以下为模拟示例，非真实文献)：
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1. **文献名称**：*Structural Insights into the ARP2/3 Complex: Role of ARPC4 in Actin Nucleation*
**作者**：Robinson RC, et al.
**摘要**：本研究通过重组表达ARPC4蛋白，结合冷冻电镜技术解析了ARP2/3复合体的三维结构，揭示了ARPC4在稳定复合体构象和促进肌动蛋白成核中的关键作用。
2. **文献名称**：*Expression and Functional Characterization of Recombinant ARPC4 in Cancer Cell Motility*
**作者**：Zhang L, et al.
**摘要**：利用大肠杆菌系统重组表达ARPC4蛋白，发现其缺失显著抑制肿瘤细胞迁移。体外实验表明，重组ARPC4可恢复ARP2/3复合体介导的肌动蛋白网络重塑。
3. **文献名称**：*ARPC4 Mutations Disrupt Actin Polymerization in Neurodevelopmental Disorders*
**作者**：Doe J, et al.
**摘要**：通过构建ARPC4重组突变体蛋白，发现其与ARP2/3复合体结合能力下降，导致肌动蛋白分支形成缺陷，为神经发育障碍的分子机制提供了实验依据。
4. **文献名称**：*High-Yield Purification of Recombinant ARPC4 for Biochemical Assays*
**作者**：Smith K, et al.
**摘要**：优化了ARPC4重组蛋白在昆虫细胞中的表达和纯化流程，获得高纯度蛋白用于体外结合实验，证实其与ARPC2亚基的相互作用依赖磷酸化修饰。
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如需真实文献，建议通过 **PubMed** 或 **Google Scholar** 检索关键词：
`ARPC4 recombinant protein`, `ARP2/3 complex structure`, `ARPC4 expression and function`。
**Background of ARPC4 Recombinant Protein**
ARPC4 (Actin-Related Protein 2/3 Complex Subunit 4) is a critical component of the Arp2/3 complex, a conserved seven-subunit protein assembly essential for regulating actin cytoskeleton dynamics. This complex nucleates branched actin filaments, enabling processes such as cell motility, membrane trafficking, and cytokinesis. ARPC4. along with ARPC2. forms the structural backbone of the Arp2/3 complex, stabilizing its conformation and facilitating interactions with actin filaments and nucleation-promoting factors (NPFs) like WASp/Scar proteins.
Recombinant ARPC4 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to study its role in actin network formation and cellular mechanics. Its purified form allows *in vitro* reconstitution of the Arp2/3 complex, enabling mechanistic insights into how actin branching is spatiotemporally controlled. Researchers employ ARPC4 recombinant protein to investigate mutations linked to diseases, such as immunodeficiencies or developmental disorders, where Arp2/3 dysfunction disrupts cytoskeletal processes. Additionally, it serves as a tool for screening therapeutic compounds targeting actin-driven pathologies, including cancer metastasis or invasive microbial infections.
Studies leveraging recombinant ARPC4 have also clarified regulatory post-translational modifications and binding partners, further elucidating how the Arp2/3 complex integrates signals from multiple pathways to coordinate cytoskeletal remodeling. Overall, ARPC4 recombinant protein is pivotal for advancing both basic and translational research in cell biology and disease mechanisms.
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