| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CbS |
| Uniprot No | P35520 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-551aa |
| 氨基酸序列 | MPSETPQAEVGPTGCPHRSGPHSAKGSLEKGSPEDKEAKEPLWIRPDAPS RCTWQLGRPASESPHHHTAPAKSPKILPDILKKIGDTPMVRINKIGKKFG LKCELLAKCEFFNAGGSVKDRISLRMIEDAERDGTLKPGDTIIEPTSGNT GIGLALAAAVRGYRCIIVMPEKMSSEKVDVLRALGAEIVRTPTNARFDSP ESHVGVAWRLKNEIPNSHILDQYRNASNPLAHYDTTADEILQQCDGKLDM LVASVGTGGTITGIARKLKEKCPGCRIIGVDPEGSILAEPEELNQTEQTT YEVEGIGYDFIPTVLDRTVVDKWFKSNDEEAFTFARMLIAQEGLLCGGSA GSTVAVAVKAAQELQEGQRCVVILPDSVRNYMTKFLSDRWMLQKGFLKEE DLTEKKPWWWHLRVQELGLSAPLTVLPTITCGHTIEILREKGFDQAPVVD EAGVILGMVTLGNMLSSLLAGKVQPSDQVGKVIYKQFKQIRLTDTLGRLS HILEMDHFALVVHEQIQYHSTGKSSQRQMVFGVVTAIDLLNFVAAQERDQ K |
| 预测分子量 | 86 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于胱硫醚β-合酶(Cystathionine Beta-Synthase, CBS)重组蛋白的参考文献概要：
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1. **文献名称**：*"Production and characterization of recombinant human cystathionine beta-synthase"*
**作者**：Kery, V., Poneleit, L., & Kraus, J.P.
**摘要**：该研究报道了在大肠杆菌中高效表达人源CBS重组蛋白的方法，通过亲和层析纯化获得高活性酶，并分析了其催化特性及辅因子(如血红素和维生素B6)对酶活性的调控作用。
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2. **文献名称**：*"Structural basis of regulation and oligomerization of human cystathionine β-synthase, the central enzyme of transsulfuration"*
**作者**：Ereno-Orbea, J., Majtan, T., Oyenarte, I., Kraus, J.P., & Martinez-Cruz, L.A.
**摘要**：通过X射线晶体学解析了重组人CBS蛋白的三维结构，揭示了其四聚体组装模式及SAM(S-腺苷甲硫氨酸)依赖的变构调控机制，为理解遗传性高胱氨酸尿症的致病突变提供结构依据。
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3. **文献名称**：*"Recombinant expression of the mammalian cystathionine beta-synthase: A multifunctional pyridoxal enzyme"*
**作者**：Taoka, S., & Banerjee, R.
**摘要**：研究比较了不同表达系统(如哺乳动物细胞和原核系统)对重组CBS蛋白功能的影响，发现辅因子结合能力与酶活性密切相关，并探讨了其抗氧化功能与硫化氢生成的关系。
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4. **文献名称**：*"Cystathionine beta-synthase mutations in homocystinuria: Functional and structural analyses"*
**作者**：Kozich, V., et al.
**摘要**：利用重组CBS蛋白技术，分析了多种临床突变体(如p.G307S)的酶活性和稳定性缺陷，验证了维生素B6治疗对部分突变体的有效性，为精准治疗提供实验依据。
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以上文献聚焦于CBS重组蛋白的表达、结构解析、功能调控及疾病相关突变研究，涵盖酶学、结构生物学和医学应用方向。
**Background of CbS Recombinant Protein**
Cystathionine beta-synthase (CbS) is a pivotal enzyme in the transsulfuration pathway, catalyzing the condensation of serine and homocysteine to form cystathionine—a critical step in hydrogen sulfide (H₂S) biosynthesis and the metabolism of sulfur-containing amino acids. CbS plays a dual role in maintaining cellular homeostasis: it regulates homocysteine levels, linked to cardiovascular and neurological health, and contributes to redox signaling via H₂S, a gaseous signaling molecule involved in inflammation, vasodilation, and apoptosis.
Genetic mutations in the *CBS* gene can lead to CBS deficiency, a rare autosomal recessive disorder characterized by hyperhomocysteinemia, which manifests as developmental delays, thromboembolism, and connective tissue abnormalities. Studying CbS function and dysfunction has been challenging due to the enzyme’s complexity, including its dependence on pyridoxal 5′-phosphate (PLP) and heme cofactors.
Recombinant CbS proteins, produced through heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells), have become indispensable tools for structural, functional, and therapeutic research. These proteins enable detailed biochemical studies, such as elucidating enzyme kinetics, mutation impacts, and cofactor interactions. Additionally, recombinant CbS supports drug discovery efforts targeting homocysteine-related disorders and H₂S-mediated pathways.
Recent advances in protein engineering and structural biology, including cryo-EM and X-ray crystallography, have unveiled conformational dynamics and regulatory mechanisms of CbS, informing the design of enzyme modulators. Recombinant CbS also holds potential for enzyme replacement therapies in genetic deficiencies. Overall, the development and application of recombinant CbS proteins continue to bridge gaps in understanding sulfur metabolism and its implications in human disease.
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