| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCN1 |
| Uniprot No | P20061 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-433aa |
| 氨基酸序列 | EICEVSEENYIRLKPLLNTMIQSNYNRGTSAVNVVLSLKLVGIQIQTLMQ KMIQQIKYNVKSRLSDVSSGELALIILALGVCRNAEENLIYDYHLIDKLE NKFQAEIENMEAHNGTPLTNYYQLSLDVLALCLFNGNYSTAEVVNHFTPE NKNYYFGSQFSVDTGAMAVLALTCVKKSLINGQIKADEGSLKNISIYTKS LVEKILSEKKENGLIGNTFSTGEAMQALFVSSDYYNENDWNCQQTLNTVL TEISQGAFSNPNAAAQVLPALMGKTFLDINKDSSCVSASGNFNISADEPI TVTPPDSQSYISVNYSVRINETYFTNVTVLNGSVFLSVMEKAQKMNDTIF GFTMEERSWGPYITCIQGLCANNNDRTYWELLSGGEPLSQGAGSYVVRNG ENLEVRWSKY |
| 预测分子量 | 62 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TCN1(转钴胺素I)重组蛋白的模拟参考文献示例，内容基于常见研究主题合理推测，建议通过学术数据库核实具体文献：
1. **标题**：*Expression and Purification of Recombinant Human TCN1 in Escherichia coli for Vitamin B12 Binding Studies*
**作者**：Zhang L, Wang Y, Chen H
**摘要**：本研究成功在大肠杆菌中表达了重组人源TCN1蛋白，并通过亲和层析纯化。实验证实重组蛋白能够有效结合维生素B12.为后续功能研究提供了工具。
2. **标题**：*Structural Characterization of Recombinant TCN1 Using Cryo-Electron Microscopy*
**作者**：Smith JR, Kumar P, Lee S
**摘要**：利用哺乳动物细胞系统表达TCN1重组蛋白，并通过冷冻电镜解析其三维结构，揭示了其与维生素B12结合的关键结构域。
3. **标题**：*Recombinant TCN1 as a Biomarker in Autoimmune Disease Diagnostics*
**作者**：Garcia M, Rodriguez A, Kim T
**摘要**：探讨重组TCN1蛋白在血清中的检测价值，发现其水平与某些自身免疫性疾病相关，提示其作为诊断标志物的潜力。
4. **标题**：*Functional Analysis of TCN1 Mutants via Recombinant Protein Expression in HEK293 Cells*
**作者**：Patel R, Nguyen V, Ito F
**摘要**：通过HEK293细胞表达突变型TCN1重组蛋白，分析其维生素B12转运功能缺陷，为遗传性钴胺素代谢异常提供机制解释。
**注意**：以上文献为示例性质，实际研究需通过PubMed、Web of Science等平台检索确认。
TCN1 (Transcobalamin I), also known as haptocorrin or R-binder, is a glycoprotein involved in the binding and transport of cobalamin (vitamin B12). It is primarily synthesized in salivary glands, gastric mucosa, and granulocytes, and is secreted into bodily fluids such as saliva, breast milk, and tears. TCN1 binds to dietary cobalamin in the upper gastrointestinal tract, protecting it from acidic degradation and facilitating its delivery to the intestine. Unlike transcobalamin II (TCN2), which mediates cellular uptake of B12. TCN1’s role is largely restricted to initial stabilization and mucosal transfer. Its exact physiological significance remains under investigation, though deficiencies or mutations are linked to metabolic disorders and hematologic abnormalities.
Recombinant TCN1 protein is engineered using expression systems like mammalian cells or *E. coli* to produce a purified, bioactive form for research and therapeutic applications. This involves cloning the TCN1 gene into expression vectors, optimizing codon usage, and employing affinity chromatography for purification. Recombinant TCN1 retains its cobalamin-binding capacity, enabling studies on B12 metabolism, autoimmune diseases (e.g., pernicious anemia), and diagnostics for cobalamin-related deficiencies. It also serves as a tool to explore TCN1’s interactions with pathogens, as some microbes exploit TCN1 for B12 acquisition. Structural studies using recombinant protein have elucidated its glycosylation patterns and binding domains, offering insights for drug design. Current challenges include scaling production while maintaining functional integrity and resolving its ambiguous role in B12 homeostasis beyond initial absorption.
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