| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP4b |
| Uniprot No | P51164 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 58-291aa |
| 氨基酸序列 | CLYVLMQTVDPYTPDYQDQLRSPGVTLRPDVYGEKGLEIVYNVSDNRTWADLTQTLHAFLAGYSPAAQEDSINCTSEQYFFQESFRAPNHTKFSCKFTADMLQNCSGLADPNFGFEEGKPCFIIKMNRIVKFLPSNGSAPRVDCAFLDQPRELGQPLQVKYYPPNGTFSLHYFPYYGKKAQPHYSNPLVAAKLLNIPRNAEVAIVCKVMAEHVTFNNPHDPYEGKVEFKLKIEK |
| 预测分子量 | 26.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP4B重组蛋白的参考文献示例(注：文献信息为模拟生成，仅供参考)：
1. **文献名称**：*"Expression and Functional Characterization of Recombinant ATP4B in HEK293 Cells"*
**作者**：Smith A, et al.
**摘要**：本研究成功在HEK293细胞中表达并纯化ATP4B重组蛋白，证实其与ATP4A亚基结合后形成功能性H+/K+-ATP酶，并参与跨膜质子转运。
2. **文献名称**：*"Structural Role of ATP4B in Gastric H+/K+-ATPase Stability"*
**作者**：Tanaka R, et al.
**摘要**：通过重组ATP4B蛋白的晶体结构分析，揭示其通过二硫键和糖基化修饰维持H+/K+-ATP酶复合体的稳定性，对胃酸分泌至关重要。
3. **文献名称**：*"ATP4B Knockout Mice Reveal Its Essential Role in Acid Secretion"*
**作者**：Wang L, et al.
**摘要**：利用重组ATP4B缺陷模型，发现其缺失导致胃壁细胞酸分泌功能丧失，证实ATP4B在酶活性和膜定位中的必要性。
4. **文献名称**：*"Recombinant ATP4B as a Therapeutic Target for Acid-Related Disorders"*
**作者**：Johnson M, et al.
**摘要**：研究重组ATP4B与抑制剂分子的相互作用，为开发靶向H+/K+-ATP酶的抗溃疡药物提供结构基础。
**建议**：如需真实文献，请通过PubMed或Google Scholar以关键词“ATP4B recombinant protein”“H+/K+-ATPase beta subunit”检索最新研究。
**Background of ATP4b Recombinant Protein**
ATP4b, also known as the beta subunit of the gastric H+/K+ ATPase, is a critical component of the proton pump responsible for acid secretion in the stomach. This enzyme, located on the apical membrane of parietal cells, exchanges cytoplasmic H+ ions with luminal K+ ions, generating the highly acidic environment necessary for digestion and pathogen defense. The ATP4b subunit plays a structural and regulatory role, stabilizing the catalytic alpha subunit (ATP4a) and ensuring proper membrane localization and function of the pump.
Recombinant ATP4b protein is produced using biotechnological methods, often expressed in heterologous systems like *E. coli* or mammalian cell lines to ensure proper folding and post-translational modifications. Its production enables detailed studies on gastric acid secretion mechanisms, autoimmune disorders (e.g., autoimmune gastritis), and interactions with therapeutic agents such as proton pump inhibitors (PPIs). In autoimmune conditions, ATP4b is a target of autoantibodies that disrupt proton pump activity, leading to hypochlorhydria and pernicious anemia.
Research applications of ATP4b recombinant protein include *in vitro* binding assays, structural analysis (e.g., crystallography), and diagnostic tool development for detecting autoantibodies. It also aids in exploring molecular pathways underlying acid-related diseases and screening potential drugs targeting acid secretion. By enabling precise manipulation and analysis, recombinant ATP4b advances both basic and clinical research, offering insights into gastric physiology and therapeutic innovations.
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