| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNFaIP2 |
| Uniprot No | Q03169 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-654aa |
| 氨基酸序列 | MSEASSEDLV PPLEAGAAPY REEEEAAKKK KEKKKKSKGL ANVFCVFTKG KKKKGQPSSA EPEDAAGSRQ GLDGPPPTVE ELKAALERGQ LEAARPLLAL ERELAAAAAA GGVSEEELVR RQSKVEALYE LLRDQVLGVL RRPLEAPPER LRQALAVVAE QEREDRQAAA AGPGTSGLAA TRPRRWLQLW RRGVAEAAEE RMGQRPAAGA EVPESVFLHL GRTMKEDLEA VVERLKPLFP AEFGVVAAYA ESYHQHFAAH LAAVAQFELC ERDTYMLLLW VQNLYPNDII NSPKLVGELQ GMGLGSLLPP RQIRLLEATF LSSEAANVRE LMDRALELEA RRWAEDVPPQ RLDGHCHSEL AIDIIQITSQ AQAKAESITL DLGSQIKRVL LVELPAFLRS YQRAFNEFLE RGKQLTNYRA NVIANINNCL SFRMSMEQNW QVPQDTLSLL LGPLGELKSH GFDTLLQNLH EDLKPLFKRF THTRWAAPVE TLENIIATVD TRLPEFSELQ GCFREELMEA LHLHLVKEYI IQLSKGRLVL KTAEQQQQLA GYILANADTI QHFCTQHGSP ATWLQPALPT LAEIIRLQDP SAIKIEVATY ATCYPDFSKG HLSAILAIKG NLSNSEVKRI RSILDVSMGA QEPSRPLFSL IKVG |
| 预测分子量 | 72,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于TNFaIP2重组蛋白研究的模拟参考文献示例(建议通过学术数据库验证并获取真实文献)：
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1. **文献名称**：*TNFaIP2 Recombinant Protein Modulates NF-κB Signaling in Inflammatory Responses*
**作者**：Zhang Y, et al.
**摘要**：研究通过大肠杆菌表达系统制备TNFaIP2重组蛋白，发现其通过抑制NF-κB通路减轻巨噬细胞的炎症反应，提示其在炎症性疾病中的潜在治疗作用。
2. **文献名称**：*Recombinant TNFaIP2 Suppresses Tumor Metastasis by Regulating Epithelial-Mesenchymal Transition*
**作者**：Lee S, Kim D.
**摘要**：利用哺乳动物细胞表达的重组TNFaIP2蛋白，在体外实验中证明其通过调控EMT相关标记物(如E-cadherin和Vimentin)抑制乳腺癌细胞迁移和侵袭。
3. **文献名称**：*Optimization of TNFaIP2 Recombinant Protein Expression and Purification in Yeast Systems*
**作者**：Chen L, et al.
**摘要**：探索在毕赤酵母中高效表达TNFaIP2重组蛋白的条件，优化纯化工艺并获得高纯度蛋白，为后续功能研究奠定基础。
4. **文献名称**：*TNFaIP2 Recombinant Protein Attenuates Autoimmune Encephalomyelitis in a Murine Model*
**作者**：Smith J, et al.
**摘要**：通过动物实验证实，注射重组TNFaIP2蛋白可减少中枢神经系统炎症细胞浸润，改善多发性硬化症模型小鼠的临床症状。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如“TNFaIP2 recombinant protein”、“TNFAIP2 expression”)获取。建议结合具体研究方向筛选近年高影响力论文。
TNFαIP2 (Tumor Necrosis Factor Alpha-Induced Protein 2), also known as MDC or MIP-1δ, is a secreted protein encoded by the TNFAIP2 gene. It was initially identified as a gene rapidly upregulated in endothelial cells and macrophages in response to pro-inflammatory cytokines like TNF-α and IL-1β. Structurally, TNFαIP2 contains conserved domains, including a cysteine-rich region and a coiled-coil domain, which mediate its interaction with extracellular matrix components and cell surface receptors. Its expression is tightly regulated by the NF-κB signaling pathway, linking it to inflammatory and immune responses.
Functionally, TNFαIP2 plays dual roles in physiological and pathological processes. It contributes to macrophage activation, angiogenesis, and tissue remodeling under inflammatory conditions. Studies suggest it enhances cell migration and proliferation while modulating apoptosis, depending on cellular context. However, dysregulated TNFαIP2 expression is implicated in chronic inflammatory diseases (e.g., atherosclerosis, rheumatoid arthritis), cancer progression (promoting tumor invasion/metastasis), and metabolic disorders. Elevated TNFαIP2 levels have been observed in several malignancies, correlating with poor prognosis.
Recombinant TNFαIP2 protein is typically produced using Escherichia coli or mammalian expression systems to ensure proper folding and post-translational modifications. It serves as a critical tool for studying TNFα-mediated signaling, screening anti-inflammatory compounds, and exploring therapeutic strategies targeting inflammation-related diseases. Recent research also highlights its potential as a diagnostic biomarker, particularly in cancers where it influences tumor microenvironment dynamics. Ongoing studies aim to clarify its receptor interactions and downstream effectors to enable targeted modulation of its pro- or anti-inflammatory effects.
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