| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSPAN1 |
| Uniprot No | O60635 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 110-211aa |
| 氨基酸序列 | YTTMAEHFLT LLVVPAIKKD YGSQEDFTQV WNTTMKGLKC CGFTNYTDFE DSPYFKENSA FPPFCCNDNV TNTANETCTK QKAHDQKVEG CFNQLLYDIR TN |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TSPAN1重组蛋白的模拟参考文献示例(注：以下内容为虚构，仅供格式参考)：
1. **《Functional characterization of recombinant TSPAN1 in cancer cell migration》**
- 作者：Zhang L, et al.
- 摘要：研究通过大肠杆菌系统表达并纯化TSPAN1重组蛋白，发现其通过调控整合素信号通路促进肺癌细胞的迁移和侵袭，为TSPAN1在肿瘤转移中的作用提供机制依据。
2. **《Structural analysis of TSPAN1 extracellular domain using recombinant protein expression in HEK293 cells》**
- 作者：Wang Y, et al.
- 摘要：利用哺乳动物HEK293细胞表达TSPAN1重组蛋白的胞外结构域，结合X射线晶体学解析其三维结构，揭示其与其他四跨膜蛋白家族成员的保守性及特异性相互作用界面。
3. **《TSPAN1 recombinant protein interacts with EGFR and modulates downstream signaling》**
- 作者：Chen H, et al.
- 摘要：通过昆虫细胞系统制备TSPAN1重组蛋白，并利用免疫共沉淀技术证实其与表皮生长因子受体(EGFR)的直接结合，进一步实验表明TSPAN1通过稳定EGFR膜定位增强MAPK信号通路活性。
4. **《Development of a TSPAN1 recombinant protein-based ELISA for autoimmune disease diagnosis》**
- 作者：Kim S, et al.
- 摘要：研究开发基于TSPAN1重组蛋白的ELISA检测方法，用于系统性红斑狼疮患者血清中自身抗体的筛查，验证其在临床诊断中的敏感性和特异性。
(注：以上文献及内容为示例性虚构，实际研究中请查阅真实数据库如PubMed或Web of Science获取权威信息。)
TSPAN1. a member of the tetraspanin superfamily, is a transmembrane protein characterized by four conserved hydrophobic domains that span the cell membrane, forming two extracellular loops and short intracellular termini. Tetraspanins are widely recognized for their role in organizing membrane microdomains, facilitating protein-protein interactions, and modulating cellular processes such as adhesion, motility, and signal transduction. TSPAN1. specifically, has been implicated in various physiological and pathological contexts, including cancer progression, immune regulation, and viral infection. Its expression is often dysregulated in malignancies, where it may influence tumor cell proliferation, metastasis, and drug resistance by interacting with integrins, growth factor receptors, or matrix metalloproteinases.
Recombinant TSPAN1 protein is engineered through heterologous expression systems, such as Escherichia coli, insect cells, or mammalian cell lines, to produce soluble or membrane-bound forms for functional studies. The recombinant version typically retains key structural features, enabling researchers to investigate its biochemical properties, ligand-binding capabilities, and post-translational modifications. Purification methods often involve affinity tags (e.g., His-tag) followed by chromatography, ensuring high purity for applications like antibody development, structural analysis, or in vitro binding assays.
Studies leveraging recombinant TSPAN1 have shed light on its role in cellular communication and disease mechanisms. For instance, it has been explored as a potential biomarker in cancers or a therapeutic target due to its involvement in oncogenic pathways. Challenges remain in mimicking its native membrane environment, as proper folding and interactions may require lipid bilayer contexts. Nevertheless, recombinant TSPAN1 remains a critical tool for dissecting its biological functions and translational potential.
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