| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DTNb |
| Uniprot No | O60941 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-627aa |
| 氨基酸序列 | MIEESGNKRK TMAEKRQLFI EMRAQNFDVI RLSTYRTACK LRFVQKRCNL HLVDIWNMIE AFRDNGLNTL DHTTEISVSR LETVISSIYY QLNKRLPSTH QISVEQSISL LLNFMIAAYD SEGRGKLTVF SVKAMLATMC GGKMLDKLRY VFSQMSDSNG LMIFSKFDQF LKEVLKLPTA VFEGPSFGYT EHSVRTCFPQ QRKIMLNMFL DTMMADPPPQ CLVWLPLMHR LAHVENVFHP VECSYCRCES MMGFRYRCQQ CHNYQLCQNC FWRGHAGGPH SNQHQMKEHS SWKSPAKKLS HAISKSLGCV PTREPPHPVF PEQPEKPLDL AHIVPPRPLT NMNDTMVSHM SSGVPTPTKR LQYSQDIPSH LADEHALIAS YVARLQHCAR VLDSPSRLDE EHRLIARYAA RLAAEAGNVT RPPTDLSFNF DANKQQRQLI AELENKNREI LQEIQRLRLE HEQASQPTPE KAQQNPTLLA ELRLLRQRKD ELEQRMSALQ ESRRELMVQL EELMKLLKEE EQKQAAQATG SPHTSPTHGG GRPMPMPVRS TSAGSTPTHC PQDSLSGVGG DVQEAFAQGT RRNLRNDLLV AADSITNTMS SLVKELHSAE EGAEEEEEKM QNGKDRG |
| 预测分子量 | 71,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DTNb重组蛋白的模拟参考文献示例，供参考：
1. **《高效表达与纯化重组人DTNb蛋白及其功能初探》**
作者：李明等
摘要：本研究成功构建了人源DTNb基因的重组质粒，并在大肠杆菌中实现可溶性表达。通过亲和层析技术纯化获得高纯度蛋白，Western blot验证其抗原性，为后续功能研究奠定基础。
2. **《DTNb重组蛋白调控骨骼肌细胞钙离子通道的机制研究》**
作者：Chen R, Wang X
摘要：利用哺乳动物细胞表达体系制备重组DTNb蛋白，发现其通过结合caveolin-3调控L型钙通道活性，揭示DTNb在肌肉收缩中的关键作用，为肌营养不良症治疗提供新靶点。
3. **《基于冷冻电镜的DTNb蛋白二聚体结构解析》**
作者：Smith J et al.
摘要：首次报道重组DTNb蛋白的3.2Å分辨率冷冻电镜结构，阐明其CC结构域介导的同源二聚化机制，突变实验验证该结构域对细胞膜锚定功能的关键影响。
4. **《DTNb重组蛋白纳米颗粒递送系统改善小鼠肌肉萎缩的研究》**
作者：张涛等
摘要：开发壳聚糖包裹的重组DTNb缓释微球，动物实验显示其能有效增加萎缩肌肉的再生标记物表达，证实蛋白递送系统在肌肉疾病治疗中的潜在应用价值。
注：以上内容为学术示例，实际引用请核实真实文献数据。
DTNb recombinant protein is a engineered form of the Disintegrin and Thrombospondin Motif (ADAMTS) family member, typically derived from human or mammalian sources through recombinant DNA technology. The "DTNb" designation often refers to specific functional domains or splice variants that retain critical biological activities. These proteins are characterized by their conserved metalloprotease domain followed by thrombospondin type-1 repeats, enabling interactions with extracellular matrix components.
Originally identified for their role in extracellular matrix remodeling, DTNb proteins regulate cell adhesion, angiogenesis, and tissue development. Their recombinant versions are optimized for research and therapeutic applications, as natural isoforms often exhibit low abundance and purification challenges. The recombinant form is commonly produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications and functional folding.
In research, DTNb recombinant proteins are used to study inflammatory diseases, cancer metastasis, and connective tissue disorders. Their thrombospondin domains show potential in modulating TGF-β signaling pathways, while the disintegrin-like regions may influence integrin-mediated cellular processes. Therapeutic exploration focuses on tissue regeneration and anti-angiogenic strategies, particularly in oncology.
Production challenges include maintaining protease activity stability and preventing autocatalytic degradation. Current versions often incorporate mutation stabilization or fusion tags while preserving native conformation. As a research tool, DTNb proteins serve as standardized reagents for binding assays, enzyme activity studies, and mechanistic investigations in developmental biology and pathology.
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