Recombinant Human P4Ha2 protein

中文名： 脯氨酰4-羟化酶α多肽Ⅱ(P4Ha2)重组蛋白
别名： P4Ha2；Prolyl 4-hydroxylase subunit alpha-2

货号: PA2000-278DB

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	P4Ha2
Uniprot No	O15460
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	22-535aa
氨基酸序列	EFFTSIGHM TDLIYAEKEL VQSLKEYILV EEAKLSKIKS WANKMEALTS KSAADAEGYL AHPVNAYKLV KRLNTDWPAL EDLVLQDSAA GFIANLSVQR QFFPTDEDEI GAAKALMRLQ DTYRLDPGTI SRGELPGTKY QAMLSVDDCF GMGRSAYNEG DYYHTVLWME QVLKQLDAGE EATTTKSQVL DYLSYAVFQL GDLHRALELT RRLLSLDPSH ERAGGNLRYF EQLLEEEREK TLTNQTEAEL ATPEGIYERP VDYLPERDVY ESLCRGEGVK LTPRRQKRLF CRYHHGNRAP QLLIAPFKEE DEWDSPHIVR YYDVMSDEEI ERIKEIAKPK LARATVRDPK TGVLTVASYR VSKSSWLEED DDPVVARVNR RMQHITGLTV KTAELLQVAN YGVGGQYEPH FDFSRNDERD TFKHLGTGNR VATFLNYMSD VEAGGATVFP DLGAAIWPKK GTAVFWYNLL RSGEGDYRTR HAACPVLVGC KWVSNKWFHE RGQEFLRPCG STEVD
预测分子量	60,9 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇关于P4HA2(Prolyl 4-Hydroxylase Alpha 2)重组蛋白的文献示例及摘要概括：

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1. **文献名称**: "Recombinant expression and functional characterization of human P4HA2 in collagen hydroxylation"

**作者**: Smith A, et al.

**摘要**: 本研究成功在大肠杆菌中表达并纯化了人源P4HA2重组蛋白，验证其与β亚基形成活性复合物的能力，证明其在体外催化胶原前体羟化中的关键作用，为研究胶原修饰机制提供工具。

2. **文献名称**: "P4HA2 overexpression promotes tumor fibrosis and metastasis in breast cancer models"

**作者**: Chen L, et al.

**摘要**: 通过重组P4HA2蛋白处理乳腺癌细胞，发现其通过增强胶原羟化促进细胞外基质重塑，加速肿瘤纤维化和转移，提示P4HA2可能成为癌症治疗的潜在靶点。

3. **文献名称**: "Structural insights into the heterodimeric assembly of P4HA2 with P4HB in hypoxia signaling"

**作者**: Tanaka K, et al.

**摘要**: 利用重组P4HA2/P4HB复合物的晶体结构分析，揭示了其在低氧条件下稳定HIF-1α的分子机制，阐明了P4HA2在细胞适应缺氧环境中的结构基础。

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注：以上文献为示例，实际研究中建议通过PubMed或Web of Science检索具体文献(关键词：P4HA2. recombinant, collagen hydroxylase)。该蛋白主要参与胶原合成调控，与纤维化疾病及癌症进展相关。

背景信息

**Background of P4Ha2 Recombinant Protein**

Prolyl 4-hydroxylase subunit alpha 2 (P4HA2) is a critical enzyme involved in the post-translational modification of collagen, the most abundant structural protein in vertebrates. As a member of the prolyl 4-hydroxylase (P4H) family, P4HA2 catalyzes the hydroxylation of proline residues in collagen precursors, a process essential for stabilizing the triple-helix structure of collagen molecules. This modification ensures proper collagen folding, secretion, and extracellular matrix (ECM) assembly, which are vital for tissue integrity, wound healing, and developmental processes.

The P4HA2 enzyme typically functions as a heterotetramer, combining two alpha subunits (P4HA2) and two beta subunits (P4HB/prolyl 4-hydroxylase beta polypeptide). Dysregulation of P4HA2 has been implicated in pathological conditions such as fibrosis, cancer metastasis, and connective tissue disorders, where abnormal collagen deposition or remodeling occurs. For instance, elevated P4HA2 expression is observed in tumor microenvironments, promoting ECM stiffness and facilitating cancer cell invasion.

Recombinant P4HA2 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce functional enzyme complexes for research and therapeutic applications. Its recombinant form retains enzymatic activity, enabling studies on collagen biosynthesis mechanisms, drug screening for P4H inhibitors, and exploration of ECM-related disease pathways. Additionally, recombinant P4HA2 serves as a tool to investigate hypoxia-inducible factor (HIF) regulation, as P4H enzymes also influence HIF-1α stability under low-oxygen conditions.

Recent advances highlight its potential as a therapeutic target, particularly in antifibrotic and anticancer strategies. By modulating P4HA2 activity, researchers aim to control excessive collagen production in fibrotic diseases or disrupt tumor ECM to enhance drug delivery. Overall, recombinant P4HA2 provides a versatile platform for both basic research and translational medicine, bridging insights into collagen biology and clinical interventions.

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