Recombinant Human gGT5 protein

中文名： γ-谷氨酰基转氨酶5(gGT5)重组蛋白
别名： gGT5；GGTLA1；Glutathione hydrolase 5 proenzyme

货号: PA2000-273DB

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	gGT5
Uniprot No	P36269
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-586aa
氨基酸序列	MARGYGATVSLVLLGLGLALAVIVLAVVLSRHQAPCGPQAFAHAAVAADSKVCSDIGRAILQQQGSPVDATIAALVCTSVVNPQSMGLGGGVIFTIYNVTTGKVEVINARETVPASHAPSLLDQCAQALPLGTGAQWIGVPGELRGYAEAHRRHGRLPWAQLFQPTIALLRGGHVVAPVLSRFLHNSILRPSLQASTLRQLFFNGTEPLRPQDPLPWPALATTLETVATEGVEVFYTGRLGQMLVEDIAKEGSQLTLQDLAKFQPEVVDALEVPLGDYTLYSPPPPAGGAILSFILNVLRGFNFSTESMARPEGRVNVYHHLVETLKFAKGQRWRLGDPRSHPKLQNASRDLLGETLAQLIRQQIDGRGDHQLSHYSLAEAWGHGTGTSHVSVLGEDGSAVAATSTINTPFGAMVYSPRTGIILNNELLDLCERCPRGSGTTPSPVSGDRVGGAPGRCWPPVPGERSPSSMVPSILINKAQGSKLVIGGAGGELIISAVAQAIMSKLWLGFDLRAAIAAPILHVNSKGCVEYEPNFSQEVQRGLQDRGQNQTQRPFFLNVVQAVSQEGACVYAVSDLRKSGEAAGY
预测分子量	62,2 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于 **GGT5(gamma-glutamyltransferase 5)重组蛋白**的3篇代表性文献及其摘要内容：

---

1. **文献名称**: *"Expression and purification of recombinant human gamma-glutamyltransferase 5 in HEK293 cells for functional studies"*

**作者**: Smith A, et al.

**摘要**: 本研究描述了在HEK293细胞中高效表达并纯化重组人源GGT5蛋白的方法，通过亲和层析和尺寸排阻色谱优化纯化流程。重组蛋白展现出与天然GGT5相似的酶活性和底物特异性，适用于后续生化及细胞功能研究。

---

2. **文献名称**: *"GGT5 promotes tumor progression via redox regulation in colorectal cancer"*

**作者**: Lee J, et al.

**摘要**: 文章揭示了重组GGT5蛋白在结直肠癌细胞中通过调节谷胱甘肽代谢和氧化应激促进肿瘤生长的机制。实验表明，重组GGT5的过表达增强了细胞的抗氧化能力，并加速了肿瘤在小鼠模型中的转移。

---

3. **文献名称**: *"Structural insights into the catalytic mechanism of human GGT5 by cryo-EM analysis"*

**作者**: Zhang Y, et al.

**摘要**: 本研究利用冷冻电镜解析了重组人源GGT5蛋白的三维结构，揭示了其底物结合口袋和催化关键残基的构象变化。研究为开发靶向GGT5的抑制剂提供了结构基础，并阐明了其与非经典谷氨酰转移酶活性的关联。

---

**备注**：以上文献信息为示例，实际文献需通过PubMed、Google Scholar等数据库检索。如需具体文章，建议结合关键词“recombinant GGT5”或“gamma-glutamyltransferase 5 expression”进一步筛选。

背景信息

gGT5 (gamma-glutamyl transferase 5) is a membrane-bound enzyme belonging to the gamma-glutamyl transferase (GGT) family, which plays a critical role in glutathione metabolism and cellular antioxidant defense. As a recombinant protein, gGT5 is produced through genetic engineering techniques, typically expressed in heterologous systems like *E. coli* or mammalian cell lines to ensure proper folding and post-translational modifications. Structurally, gGT5 exists as a heterodimer composed of a heavy chain (responsible for anchoring to the membrane) and a light chain (catalytic subunit), processed from a single precursor protein.

Functionally, gGT5 catalyzes the transfer of gamma-glutamyl groups from glutathione to amino acids or peptides, facilitating glutathione recycling and amino acid transport. This activity is vital for maintaining redox homeostasis, detoxification processes, and regulating oxidative stress. Dysregulation of gGT5 has been implicated in pathological conditions, including cancer progression (by promoting tumor cell survival in oxidative environments), neurodegenerative diseases, and drug-induced organ toxicity.

Recombinant gGT5 is widely used in biochemical research to study enzyme kinetics, substrate specificity, and inhibitor screening. It also serves as a tool for developing diagnostic assays and therapeutic agents targeting glutathione-related disorders. In biopharmaceutical production, recombinant gGT5 aids in optimizing protein expression systems by enhancing redox conditions. Quality control of the recombinant protein involves rigorous validation of enzymatic activity, purity, and structural integrity through techniques like SDS-PAGE, Western blotting, and mass spectrometry. Its applications extend to industrial biocatalysis and environmental biotechnology for degrading glutathione-conjugated pollutants.