| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHgA2 |
| Uniprot No | Q9Y5I4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-884aa |
| 氨基酸序列 | MEQAGTRPAATEHPRLRRPMPWLLLLPLLLLLLLLLPGPAASQLRYSVPE EQAPGALVGNVARALGLELRRLGPGCLRINHLGAPSPRYLELDLTSGALF VNERIDREALCEQRPRCLLSLEVLAHNPVAVSAVEVEILDINDNSPRFPR PNYQLQVSESVAPGARFHIESAQDPDVGANSVQTYELSPSEHFELDLKPL QENSKVLELVLRKGLDREQAALHHLVLTAVDGGIPARSGTAQISVRVLDT NDNSPAFDQSTYRVQLREDSPPGTLVVKLNASDPDEGSNGELRYSLSSYT SDRERQLFSIDASTGEVRVIGGLDYEEASSYQIYVQATDRGPVPMAGHCK VLVDIVDVNDNAPEVVLTDLYSPVPENATPNTIVAVLSVNDQDSGPNRKV SLGLEATLPFRLNGFGNSYTLVVSGPLDRERVAVYNITVTATDGGIPQLT SLRTLKVEISDINDNPPSFLEDSYSIYIQENNLPGVLLCTVQATDPDEKE NAEVTYSLLEREIQGLPVTSYVSINSASGSLYAVNSFDYEKFREFFVTVE AQDKGSPPLSSTVTANVYVVDMNDHAPHILYPTSTNSSAAFEMVPRTAPA GYLVTKVIAMDSDSGQNAWLFYHLAQTSDLDLFKVELHTGEIRTTRKMGD ESGSTFNLTVVVRDNGEPSLSASVAITVAVVDRVSKILPDTQRHVKSPRT YSEITLYLIIALSTVSFIFLLTIIILSIIKCYRYTAYGTACCGGFCGVRE RSPAELYKQANNNIDARIPHGLKVQPHFIEVRGNGSLTKTYCYKACLTAG SGSDTFMFYNTGAQTGPGPSGAQAAVTDSRNLTGQSGQNAGNLIILKNEA VSQNEVRQWSGGLLQTHAFVTHPPISCDLALLSH |
| 预测分子量 | 109 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性的参考文献示例，涵盖PCDHgA2重组蛋白的不同研究方向：
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1. **文献名称**：*Expression and Purification of Recombinant PCDHgA2 in E. coli for Structural Studies*
**作者**：Zhang, Y. et al.
**摘要**：本研究报道了通过大肠杆菌表达系统高效生产PCDHgA2重组蛋白的优化策略，采用His标签亲和层析纯化，并通过Western blot验证蛋白特异性，为后续功能研究提供材料基础。
2. **文献名称**：*Structural Insights into PCDHgA2 Extracellular Domain and Its Role in Neural Adhesion*
**作者**：Li, X. & Wang, H.
**摘要**：通过X射线晶体学解析PCDHgA2胞外结构域的三维结构，揭示其独特的钙离子结合位点，并证明该结构对神经元细胞间黏附能力的调控作用。
3. **文献名称**：*Functional Analysis of PCDHgA2 in Synaptic Specificity Using Recombinant Protein-based Assays*
**作者**：Chen, J. et al.
**摘要**：利用重组PCDHgA2蛋白进行体外神经元共培养实验，发现其选择性促进海马神经元突触形成，提示其在神经回路特异性连接中的关键功能。
4. **文献名称**：*Development of a PCDHgA2-Specific ELISA for Autoantibody Detection in Neurological Disorders*
**作者**：Kim, S. et al.
**摘要**：基于重组PCDHgA2蛋白建立高灵敏度ELISA检测方法，首次在部分自身免疫性脑炎患者血清中发现抗PCDHgA2抗体，为疾病诊断提供新标志物。
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注：以上文献为示例性内容，实际研究中请以真实发表的论文为准。
**Background of PCDHgA2 Recombinant Protein**
The protocadherin gamma subfamily A2 (PCDHgA2) is a member of the clustered protocadherin-γ (PCDH-γ) family, a group of transmembrane proteins critical for neural development, synaptic connectivity, and cell-cell recognition in the central nervous system. Protocadherins are adhesion molecules that mediate homophilic interactions, playing roles in neuronal circuit assembly, axon guidance, and dendritic self-avoidance. The PCDH-γ cluster contains 22 variable exons (γA1–γA12 and γB1–γB10) and a set of constant exons. PCDHgA2. encoded by the γA2 variable exon, is one of the "alternatively spliced" isoforms that combine with constant exons to generate diversity in extracellular domains, enabling precise neural wiring.
Recombinant PCDHgA2 protein is engineered in vitro, typically using mammalian or bacterial expression systems, to study its structural and functional properties. It retains key domains, including six extracellular cadherin repeats (EC1–EC6) involved in calcium-dependent adhesion, a transmembrane domain, and a cytoplasmic region that interacts with intracellular signaling partners. Researchers often produce tagged versions (e.g., His-tag, GST-tag) for purification and detection.
Studies using PCDHgA2 recombinant protein focus on its role in synapse formation, neuronal survival, and synaptic plasticity. Dysregulation of PCDH-γ genes, including PCDHgA2. has been linked to neurodevelopmental disorders (e.g., autism, epilepsy) and cancers. Recombinant PCDHgA2 also serves as a tool to investigate homophilic binding specificity, signaling pathways (e.g., Wnt/β-catenin), and potential therapeutic targets. Its in vitro applications include cell adhesion assays, protein interaction studies, and antibody development.
Overall, PCDHgA2 recombinant protein provides a versatile platform to decode the molecular mechanisms of neural development and disease.
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