| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCNN1a |
| Uniprot No | P37088 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-669aa |
| 氨基酸序列 | MEGNKLEEQDSSPPQSTPGLMKGNKREEQGLGPEPAAPQQPTAEEEALIEFHRSYRELFEFFCNNTTIHGAIRLVCSQHNRMKTAFWAVLWLCTFGMMYWQFGLLFGEYFSYPVSLNINLNSDKLVFPAVTICTLNPYRYPEIKEELEELDRITEQTLFDLYKYSSFTTLVAGSRSRRDLRGTLPHPLQRLRVPPPPHGARRARSVASSLRDNNPQVDWKDWKIGFQLCNQNKSDCFYQTYSSGVDAVREWYRFHYINILSRLPETLPSLEEDTLGNFIFACRFNQVSCNQANYSHFHHPMYGNCYTFNDKNNSNLWMSSMPGINNGLSLMLRAEQNDFIPLLSTVTGARVMVHGQDEPAFMDDGGFNLRPGVETSISMRKETLDRLGGDYGDCTKNGSDVPVENLYPSKYTQQVCIHSCFQESMIKECGCAYIFYPRPQNVEYCDYRKHSSWGYCYYKLQVDFSSDHLGCFTKCRKPCSVTSYQLSAGYSRWPSVTSQEWVFQMLSRQNNYTVNNKRNGVAKVNIFFKELNYKTNSESPSVTMVTLLSNLGSQWSLWFGSSVLSVVEMAELVFDLLVIMFLMLLRRFRSRYWSPGRGGRGAQEVASTLASSPPSHFCPHPMSLSLSQPGPAPSPALTAPPPAYATLGPRPSPGGSAGASSSTCPLGGP |
| 预测分子量 | 75,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCNN1a(α-ENaC)重组蛋白的3篇参考文献示例(注：文献为模拟示例，实际引用需核实原文)：
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1. **文献名称**: *"Functional Expression of Recombinant Human α-ENaC in Xenopus Oocytes"*
**作者**: Smith JL, et al.
**摘要**: 该研究通过将人源SCNN1a基因克隆至非洲爪蟾卵母细胞中，成功表达功能性α-ENaC重组蛋白。电生理分析表明，该重组通道具有典型的钠离子选择性及阿米洛利敏感性，为研究ENaC的调控机制提供了模型。
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2. **文献名称**: *"Purification and Structural Characterization of α-ENaC Recombinant Protein for Drug Screening"*
**作者**: Chen R, et al.
**摘要**: 研究团队利用昆虫细胞表达系统纯化出高纯度SCNN1a重组蛋白，并通过冷冻电镜解析其三维结构。该蛋白被用于高通量药物筛选，以发现针对囊性纤维化或高血压的新型ENaC调节剂。
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3. **文献名称**: *"Role of α-ENaC in Airway Surface Hydration: Insights from Recombinant Protein Studies"*
**作者**: Gupta S, et al.
**摘要**: 通过体外重建α-ENaC重组蛋白的离子通道功能，研究证实其在呼吸道液体平衡中的作用。过度活跃的SCNN1a会导致气道表面脱水，为慢性阻塞性肺病(COPD)的病理机制提供了分子层面的解释。
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如需实际文献，建议通过PubMed或Google Scholar搜索关键词：**SCNN1a recombinant protein、α-ENaC expression、epithelial sodium channel purification**。
**Background of SCNN1a Recombinant Protein**
SCNN1a (sodium channel epithelial 1 alpha subunit) encodes the α-subunit of the epithelial sodium channel (ENaC), a critical regulator of sodium transport across epithelial tissues. ENaC, composed of α, β, γ (and sometimes δ) subunits, mediates electrogenic Na⁺ reabsorption in organs such as the kidneys, lungs, and colon, playing a pivotal role in fluid homeostasis, blood pressure regulation, and airway surface liquid maintenance. Dysfunctional ENaC activity is linked to pathologies like Liddle syndrome (hypertension), pseudohypoaldosteronism type 1 (salt-wasting), and cystic fibrosis (due to secondary ENaC overactivation).
The SCNN1a recombinant protein is engineered to study ENaC structure-function relationships, ion selectivity, and regulatory mechanisms. Produced via heterologous expression systems (e.g., mammalian cells, *Xenopus* oocytes), recombinant SCNN1a retains post-translational modifications essential for channel assembly and trafficking. Researchers employ it to investigate ENaC interactions with hormones (e.g., aldosterone), proteases (e.g., furin), and pharmacological agents (e.g., amiloride, a diuretic targeting ENaC).
Its applications extend to drug discovery for hypertension, cystic fibrosis, and edema. Mutagenesis studies using recombinant SCNN1a help identify residues critical for ion conduction, gating, or disease-associated mutations. Additionally, structural analyses (cryo-EM, X-ray crystallography) rely on purified recombinant subunits to resolve ENaC's architecture and activation mechanisms.
In summary, SCNN1a recombinant protein serves as a vital tool for dissecting ENaC biology and developing therapies targeting sodium transport disorders.
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