| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTDSP1 |
| Uniprot No | Q9GZU7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-260aa |
| 氨基酸序列 | MDSSAVITQI SKEEARGPLR GKGDQKSAAS KPRSRGILH SLFCCVCRDD GEALPAHSGA PLLVEENGAI PKTPVQYLLP EAKAQDSDKI VVIDLDETL VHSSFKPVNN ADFIIPVEID GVVHQVYVLK RPHVDEFLQR MGELFECVLF TASLAKYADP VADLLDKWGA FRARLFRESC VFHRGNYVKD LSRLGRDLRR VLILDNSPAS VFHPDNAVP VASWFDNMSD TELHDLLPFF EQLSRVDDVY SVLRQPRPGS |
| 预测分子量 | 56 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CTDSP1重组蛋白的3篇示例文献摘要(注：文献信息为模拟生成，仅供参考)：
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1. **文献名称**：*"Expression and functional analysis of recombinant CTDSP1 in transcriptional regulation"*
**作者**：Zhang Y, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化重组CTDSP1蛋白，证实其具有特异性去磷酸化RNA聚合酶II C端结构域(CTD)的Ser2磷酸化位点的活性，并揭示其通过调控转录终止影响基因表达。
2. **文献名称**：*"Structural characterization of CTDSP1 phosphatase and its interaction with RNA Pol II"*
**作者**：Lee S, et al.
**摘要**：通过X射线晶体学解析重组人源CTDSP1蛋白的三维结构，发现其催化结构域的关键氨基酸残基对底物结合至关重要，并利用体外酶活实验验证其底物特异性。
3. **文献名称**：*"CTDSP1-mediated dephosphorylation in BMP/Smad signaling pathway"*
**作者**：Wang H, et al.
**摘要**：在HEK293细胞中过表达重组CTDSP1.证明其通过去磷酸化Smad1蛋白负调控BMP信号通路，影响细胞分化过程，为CTDSP1在发育中的作用提供新证据。
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如需查找真实文献，建议通过PubMed或Google Scholar搜索关键词“CTDSP1 recombinant protein”或“CTDSP1 phosphatase expression”。
CTDSP1 (CTD small phosphatase 1) is a protein phosphatase belonging to the FCP1-type phosphatase family, primarily involved in regulating RNA polymerase II (RNAPII) activity through dephosphorylation of the C-terminal domain (CTD) of its largest subunit. The CTD, composed of heptapeptide repeats, undergoes dynamic phosphorylation cycles during transcription, which are critical for coordinating transcription initiation, elongation, and termination. CTDSP1 specifically targets phosphorylated serine residues (e.g., Ser5 and Ser2), modulating RNAPII processivity and interactions with transcriptional machinery. This enzyme plays a role in gene expression regulation, cell cycle progression, and cellular differentiation.
Recombinant CTDSP1 protein is engineered for in vitro studies to dissect its biochemical properties and molecular mechanisms. It is typically expressed in prokaryotic (e.g., *E. coli*) or eukaryotic systems to ensure proper folding and post-translational modifications. The recombinant form retains catalytic activity, enabling researchers to study its substrate specificity, kinetics, and interactions with regulatory partners (e.g., transcription factors or other phosphatases/kinases). Structurally, CTDSP1 contains a conserved phosphatase domain and nuclear localization signals, reflecting its role in nuclear transcriptional regulation.
Research on CTDSP1 has implications in cancer biology, as dysregulation of CTD phosphorylation is linked to oncogenesis and metastasis. It also intersects with developmental pathways, such as TGF-β signaling, where it may dephosphorylate SMAD transcription factors. Additionally, CTDSP1 is implicated in neuronal development and diseases, including glioblastoma. Recombinant CTDSP1 serves as a tool for drug discovery, particularly in designing inhibitors to target CTD phosphatase activity in pathological conditions. Its study advances understanding of post-transcriptional regulation and offers potential therapeutic avenues for diseases tied to transcriptional dysregulation.
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