| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTF1a |
| Uniprot No | Q7RTS3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-328aa |
| 氨基酸序列 | MDAVLLEHFP GGLDAFPSSY FDEDDFFTDQ SSRDPLEDGD ELLADEQAEV EFLSHQLHEY CYRDGACLLL QPAPPAAPLA LAPPSSGGLG EPDDGGGGGY CCETGAPPGG FPYSPGSPPS CLAYPCAGAA VLSPGARLRG LSGAAAAAAR RRRRVRSEAE LQQLRQAANV RERRRMQSIN DAFEGLRSHI PTLPYEKRLS KVDTLRLAIG YINFLSELVQ ADLPLRGGGA GGCGGPGGGG RLGGDSPGSQ AQKVIICHRG TRSPSPSDPD YGLPPLAGHS LSWTDEKQLK EQNIIRTAKV WTPEDPRKLN SKSSFNNIEN EPPFEFVS |
| 预测分子量 | 34,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTF1a重组蛋白的示例参考文献(内容为虚拟示例，仅供参考)：
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1. **文献名称**：*"Recombinant PTF1a Protein Enhances Pancreatic Progenitor Cell Differentiation In Vitro"*
**作者**：Smith J, et al.
**摘要**：研究利用大肠杆菌表达系统成功纯化PTF1a重组蛋白，并通过体外实验证明其可激活胰腺发育相关基因(如Pdx1、Ngn3)，促进胚胎干细胞向胰腺祖细胞分化。
2. **文献名称**：*"Functional Characterization of PTF1a in Acinar Cell Development Using Recombinant Protein Delivery"*
**作者**：Chen L, et al.
**摘要**：通过真核表达体系获得高活性PTF1a重组蛋白，发现其直接结合CPA1基因启动子区域，调控腺泡细胞特异性酶的表达，为胰腺外分泌功能障碍治疗提供依据。
3. **文献名称**：*"PTF1a Recombinant Protein Reprograms Human Fibroblasts into Pancreatic Lineage Cells"*
**作者**：Yamamoto K, et al.
**摘要**：将PTF1a重组蛋白与染色质修饰因子联合导入成纤维细胞，诱导其转分化为胰岛素分泌细胞，证实PTF1a在细胞重编程中的关键作用。
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**注**：以上为模拟文献，实际研究中建议通过PubMed或Web of Science检索真实文献(关键词：PTF1a recombinant protein, pancreatic differentiation, gene regulation)。
PTF1a (Pancreas-specific transcription factor 1a) is a critical transcriptional regulator essential for pancreatic development and cell lineage specification. Encoded by the *PTF1A* gene in humans, this protein belongs to the basic helix-loop-helix (bHLH) family of transcription factors. It plays a pivotal role in determining pancreatic progenitor cell fate, particularly during embryogenesis, by promoting the differentiation of exocrine cells (e.g., acinar cells) and a subset of endocrine cells. PTF1a forms a trimeric complex with RBP-Jκ (a Notch signaling mediator) and E12/E47. enabling DNA binding and activation of target genes such as *CPA1*, *CEL*, and *AMY2A*, which are crucial for digestive enzyme production.
Recombinant PTF1a protein is engineered for research applications, often produced in bacterial or mammalian expression systems with purification tags (e.g., His-tag) to facilitate isolation. Its production enables studies on pancreatic development, regeneration, and disease mechanisms. For instance, PTF1a dysfunction is linked to pancreatic agenesis, neonatal diabetes, and pancreatic cancer. Researchers use recombinant PTF1a to investigate its role in reprogramming non-pancreatic cells into insulin-producing β-cells or acinar-like cells, offering potential therapeutic avenues for diabetes or exocrine insufficiency. Additionally, it serves as a tool to model genetic mutations or screen compounds affecting pancreatic differentiation. The protein’s structure-function relationships, post-translational modifications, and interaction networks remain active areas of study, bridging developmental biology and translational medicine.
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