| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTBP1 |
| Uniprot No | Q13363 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-440aa |
| 氨基酸序列 | MGSSHLLNKG LPLGVRPPIM NGPLHPRPLV ALLDGRDCTV EMPILKDVAT VAFCDAQSTQ EIHEKVLNEA VGALMYHTIT LTREDLEKFK ALRIIVRIGS GFDNIDIKSA GDLGIAVCNV PAASVEETAD STLCHILNLY RRATWLHQAL REGTRVQSVE QIREVASGAA RIRGETLGII GLGRVGQAVA LRAKAFGFNV LFYDPYLSDG VERALGLQRV STLQDLLFHS DCVTLHCGLN EHNHHLINDF TVKQMRQGAF LVNTARGGLV DEKALAQALK EGRIRGAALD VHESEPFSFS QGPLKDAPNL ICTPHAAWYS EQASIEMREE AAREIRRAIT GRIPDSLKNC VNKDHLTAAT HWASMDPAVV HPELNGAAYR YPPGVVGVAP TGIPAAVEGI VPSAMSLSHG LPPVAHPPHA PSPGQTVKPE ADRDHASDQL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CTBP1重组蛋白的模拟参考文献示例(非真实文献，供参考格式)：
1. **标题**: "Recombinant CTBP1 Expression and Purification in E. coli for Functional Studies"
**作者**: Smith A, et al.
**摘要**: 该研究利用大肠杆菌表达系统成功表达并纯化了重组人源CTBP1蛋白，优化了纯化条件以获得高纯度蛋白，并通过圆二色光谱验证了其二级结构完整性，为后续体外功能研究奠定基础。
2. **标题**: "Structural Insights into CTBP1-Mediated Transcriptional Repression"
**作者**: Johnson R, et al.
**摘要**: 通过X射线晶体学解析了重组CTBP1蛋白的晶体结构，揭示了其NAD+结合域的关键构象变化，并证明该结构变化对CTBP1招募组蛋白去乙酰化酶(HDACs)及抑制靶基因转录具有调控作用。
3. **标题**: "CTBP1 Interaction with Oncogenic Proteins in Colorectal Cancer Models"
**作者**: Lee H, et al.
**摘要**: 利用重组CTBP1蛋白进行体外结合实验，发现其与β-catenin和APC蛋白存在直接相互作用，并证实CTBP1过表达通过Wnt信号通路促进结直肠癌细胞增殖，提示其作为潜在治疗靶点。
4. **标题**: "Development of a CTBP1 Inhibitor Screening Platform Using Recombinant Protein"
**作者**: Garcia M, et al.
**摘要**: 基于重组CTBP1蛋白建立高通量药物筛选系统，筛选出小分子化合物NSC2115可竞争性结合CTBP1的底物结合位点，抑制其转录共抑制功能，为癌症治疗提供新策略。
(注：以上文献为虚构内容，实际研究中请通过PubMed或Web of Science等数据库检索真实文献。)
**Background of CTBP1 Recombinant Protein**
CTBP1 (C-terminal Binding Protein 1) is a transcriptional corepressor that plays a critical role in regulating gene expression by mediating chromatin remodeling and epigenetic modifications. It interacts with diverse transcription factors, including E1A, tumor suppressors, and oncoproteins, to repress target genes involved in apoptosis, differentiation, and cell cycle control. CTBP1’s activity is tightly linked to cellular metabolism, as it binds NADH/NAD+ through its dehydrogenase-like domain, enabling metabolic sensing to modulate its transcriptional regulatory functions. Dysregulation of CTBP1 is implicated in cancer progression, metastasis, and therapy resistance, owing to its ability to suppress pro-apoptotic genes and enhance survival pathways.
Recombinant CTBP1 protein is engineered *in vitro* using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for mechanistic studies. It retains key structural features, including the N-terminal PXDLS-binding domain for partner recruitment and the C-terminal dehydrogenase domain essential for dimerization and co-repressor complex assembly (e.g., HDACs, Polycomb group proteins). Researchers utilize CTBP1 recombinant protein to investigate its biochemical interactions, enzymatic properties, and role in chromatin remodeling via *in vitro* assays, such as pull-down experiments, chromatin immunoprecipitation (ChIP), or enzymatic activity screens.
Additionally, CTBP1 recombinant protein serves as a tool for drug discovery, particularly in identifying inhibitors that disrupt its dimerization or interactions with oncogenic partners. Its involvement in metabolic adaptation and tumorigenesis highlights its potential as a therapeutic target. Studies also explore its contributions to non-cancer pathologies, including metabolic syndromes and neurodegenerative diseases, underscoring its broad regulatory influence. Overall, CTBP1 recombinant protein is vital for dissecting molecular pathways in development, disease, and cellular homeostasis.
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