| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IkBa |
| Uniprot No | P25963 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317aa |
| 氨基酸序列 | MFQAAERPQE WAMEGPRDGL KKERLLDDRH DSGLDSMKDE EYEQMVKELQ EIRLEPQEVP RGSEPWKQQL TEDGDSFLHL AIIHEEKALT MEVIRQVKGD LAFLNFQNNL QQTPLHLAVI TNQPEIAEAL LGAGCDPELR DFRGNTPLHL ACEQGCLASV GVLTQSCTTP HLHSILKATN YNGHTCLHLA SIHGYLGIVE LLVSLGADVN AQEPCNGRTA LHLAVDLQNP DLVSLLLKCG ADVNRVTYQG YSPYQLTWGR PSTRIQQQLG QLTLENLQML PESEDEESYD TESEFTEFTE DELPYDDCVF GGQRLTL |
| 预测分子量 | 35,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为基于IkBα重组蛋白常见研究方向的示例文献(模拟构造，仅供参考)：
1. **文献名称**：*"Purification and characterization of recombinant IkBα for structural analysis"*
**作者**：Chen, L., & Baldwin, A. S.
**摘要**：该研究报道了通过大肠杆菌表达系统高效表达并纯化重组IkBα蛋白，利用X射线晶体学解析其结构，揭示了其与NF-κB p50/p65亚基相互作用的分子机制。
2. **文献名称**：*"Functional analysis of IkBα phosphorylation mutants in NF-κB signaling"*
**作者**：Hayden, M. S., & Ghosh, S.
**摘要**：通过构建IkBα磷酸化位点突变体重组蛋白，验证其Ser32/Ser36位点的磷酸化对NF-κB激活的关键作用，并证明其在炎症反应中的调控功能。
3. **文献名称**：*"Recombinant IkBα inhibits TNF-α-induced NF-κB activation in vitro"*
**作者**：Karin, M., & Ben-Neriah, Y.
**摘要**：体外实验表明，外源添加重组IkBα蛋白可竞争性抑制TNF-α诱导的NF-κB核转位，为开发基于IkBα的抗炎疗法提供理论依据。
4. **文献名称**：*"Stability and degradation kinetics of recombinant IkBα in cellular systems"*
**作者**：Verma, I. M., et al.
**摘要**：研究重组IkBα蛋白在细胞内的稳定性，发现其通过泛素-蛋白酶体途径快速降解的动态过程，并探讨了其在癌症模型中的潜在应用。
---
**建议**：实际文献可通过PubMed、Google Scholar等平台以关键词“recombinant IkBα”、“IkBα expression”或“NF-kB signaling”检索。
**Background of IκBα Recombinant Protein**
IκBα (Inhibitor of κB alpha) is a critical regulatory protein in the NF-κB signaling pathway, which governs cellular responses to inflammation, immune activation, and stress. Under resting conditions, IκBα binds to NF-κB transcription factors (e.g., p50/p65 heterodimers), sequestering them in the cytoplasm and blocking their DNA-binding activity. Upon stimulation by cytokines, pathogens, or stress signals, IκBα undergoes phosphorylation by the IκB kinase (IKK) complex, leading to its ubiquitination and proteasomal degradation. This releases NF-κB, allowing its nuclear translocation and activation of target genes involved in immunity, apoptosis, and proliferation.
Recombinant IκBα proteins are engineered *in vitro* using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His, GST) for purification and detection. These proteins retain functional domains, including the N-terminal phosphorylation sites (Ser32/36) and the C-terminal PEST domain, which regulate stability and degradation. Researchers utilize recombinant IκBα to study NF-κB dynamics, such as pathway inhibition, protein-protein interactions, or reconstitution experiments in IκBα-deficient cells.
Applications include *in vitro* kinase assays to study IKK activity, screening small molecules that modulate NF-κB signaling, and serving as controls in Western blotting or EMSA. Mutant variants (e.g., phosphorylation-resistant S32A/S36A) are also generated to dissect signaling mechanisms. Quality control involves verifying purity (>90% by SDS-PAGE) and functionality (e.g., binding to NF-κB in pull-down assays). Overall, recombinant IκBα is a pivotal tool for unraveling NF-κB-related pathologies, including cancer, autoimmune diseases, and chronic inflammation.
×
