| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CST5 |
| Uniprot No | P28325 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-142aa |
| 氨基酸序列 | GSASAQSRTLAGGIHATDLNDKSVQCALDFAISEYNKVINKDEYYSRPLQ VMAAYQQIVG GVNYYFNVKFGRTTCTKSQPNLDNCPFNDQPKLKEEEF CSFQINEVPWEDKISILNYKCR KV |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**: "Recombinant human cystatin 5 inhibits cathepsin B and suppresses tumor cell migration"
**作者**: Zhang Y, et al.
**摘要**: 该研究报道了重组人CST5蛋白的表达及功能，证实其通过抑制组织蛋白酶B的活性，有效减少肿瘤细胞的迁移能力，为癌症治疗提供潜在靶点。
2. **文献名称**: "Expression and purification of biologically active recombinant cystatin 5 in insect cells"
**作者**: Lee S, Kim D.
**摘要**: 研究者利用昆虫细胞系统高效表达重组CST5.并通过体外实验验证其抑制半胱氨酸蛋白酶的活性，优化了纯化工艺以提高产量。
3. **文献名称**: "Cystatin 5 recombinant protein attenuates neuroinflammation in a mouse model of Alzheimer’s disease"
**作者**: Chen L, et al.
**摘要**: 研究通过动物模型证明，重组CST5蛋白可减少β-淀粉样蛋白诱导的神经炎症反应，提示其在神经退行性疾病中的治疗潜力。
4. **文献名称**: "Structural insights into the inhibitory mechanism of cystatin 5 against legumain protease"
**作者**: Wang H, et al.
**摘要**: 该文献解析了重组CST5与蛋白酶legumain的复合物晶体结构，阐明了其特异性抑制机制，为设计相关酶抑制剂提供结构基础。
(注：以上文献为示例，实际引用需根据具体论文调整。)
**Background of CST5 Recombinant Protein**
CST5 (Cystatin 5), also known as Cystatin-related epididymal spermatogenic protein (CRES), is a member of the cystatin superfamily, a group of cysteine protease inhibitors involved in regulating proteolytic activity. Unlike typical cystatins, CST5 is classified as a type 2 cystatin family member but exhibits distinct tissue-specific expression, primarily in the male reproductive system, including the epididymis and testes. It plays a critical role in sperm maturation and fertility by modulating protease-mediated processes during spermatogenesis and epididymal transit.
Structurally, CST5 retains the conserved cystatin-fold domain but lacks a signal peptide, suggesting unique regulatory mechanisms. Its inhibitory activity targets cathepsins, a class of lysosomal proteases, thereby influencing extracellular matrix remodeling, immune responses, and cellular homeostasis. Dysregulation of CST5 has been linked to male infertility, inflammation, and certain cancers, highlighting its physiological and pathological relevance.
Recombinant CST5 protein is produced using biotechnological platforms (e.g., *E. coli* or mammalian expression systems) to ensure high purity and bioactivity. Its applications span *in vitro* studies exploring protease-inhibitor interactions, drug discovery for fertility disorders, and biomarker research. Additionally, recombinant CST5 serves as a tool to dissect molecular pathways in reproductive biology and immune regulation. Recent studies also investigate its potential in cancer therapy, given its role in modulating tumor-associated proteolysis.
Overall, CST5 recombinant protein bridges fundamental research and translational medicine, offering insights into protease regulation and therapeutic targeting.
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