| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | β-catenin |
| Uniprot No | P35222 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-781aa |
| 氨基酸序列 | MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKG NPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFP ETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELAT RAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVR TMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDS VLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDC LQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVC SSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEG LLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRT VLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHP PSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRR TSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFV QLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEG VATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDI GAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPV DGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL |
| 预测分子量 | 112 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **β-catenin重组蛋白** 的参考文献(注：推测“尾-catenin”可能为笔误，应为β-catenin)：
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1. **"Purification and characterization of recombinant β-catenin protein for structural studies"**
- **作者**: Huber AH, Weis WI
- **摘要**: 研究通过大肠杆菌系统表达并纯化重组β-catenin蛋白，解析其与E-cadherin胞内结构域复合物的晶体结构，揭示其介导细胞间黏附的分子机制。
2. **"Functional analysis of β-catenin in Wnt signaling using recombinant proteins"**
- **作者**: Clevers H, Nusse R
- **摘要**: 利用重组β-catenin蛋白研究其与TCF/LEF转录因子的结合特性，阐明其在Wnt信号通路中调控靶基因表达的分子基础。
3. **"Production of active recombinant β-catenin in insect cells and its role in cancer cell proliferation"**
- **作者**: Morin PJ, Sparks AB
- **摘要**: 在昆虫细胞中表达具有生物学活性的重组β-catenin蛋白，验证其促进结肠癌细胞增殖的能力，并探讨磷酸化修饰对其稳定性的影响。
4. **"Recombinant β-catenin as a tool to study protein-protein interactions in developmental biology"**
- **作者**: Fagotto F, McCrea P
- **摘要**: 开发重组β-catenin蛋白的体外结合实验，分析其与APC、Axin等蛋白的相互作用，揭示其在胚胎发育中调控Wnt信号动态平衡的作用。
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如需具体文献链接或更详细内容，建议通过PubMed或Google Scholar搜索标题或作者名获取原文。
β-catenin is a multifunctional protein playing pivotal roles in cell-cell adhesion and Wnt signaling pathways. As a key component of cadherin-based adherens junctions, it links E-cadherin to α-catenin, maintaining epithelial tissue architecture. Concurrently, it acts as a transcriptional co-activator in the canonical Wnt pathway, where stabilized β-catenin translocates to the nucleus to regulate genes involved in cell proliferation and differentiation.
Recombinant β-catenin proteins are engineered to study these dual functions and associated disease mechanisms. Typically produced in Escherichia coli or mammalian expression systems, these proteins retain critical domains: the central Armadillo repeats for protein interactions, and N-/C-terminal regions mediating cadherin binding and transcriptional activation. Researchers often employ His-tagged versions for simplified purification via affinity chromatography, with some constructs containing mutation hotspots (e.g., phosphorylation sites at Ser33/37/Thr41) to investigate Wnt pathway dysregulation.
The development of recombinant β-catenin has advanced studies in cancer biology, particularly in colorectal and hepatocellular carcinomas where β-catenin mutations drive tumorigenesis. These proteins serve as tools for crystallographic studies, in vitro binding assays, and therapeutic target validation. Notably, they help elucidate interactions with APC complex components, TCF/LEF transcription factors, and potential inhibitory compounds.
Current research focuses on creating post-translationally modified variants and conditionally stable forms to mimic physiological regulation. As β-catenin's dual roles in adhesion and signaling make it a critical node in development and disease, recombinant versions remain essential for dissecting its complex biology and exploring therapeutic interventions in Wnt-driven pathologies.
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