| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSNK2A2 |
| Uniprot No | P19784 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 8-350aa |
| 氨基酸序列 | RARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSAN |
| 预测分子量 | 56.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSNK2A2(酪蛋白激酶2α2亚基)重组蛋白的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*Expression and purification of recombinant human CSNK2A2 in Escherichia coli for structural studies*
**作者**：Zhang L, et al.
**摘要**：本研究成功构建了人源CSNK2A2的重组表达载体，利用大肠杆菌系统实现了该蛋白的可溶性表达，并通过亲和层析和凝胶过滤技术纯化获得高纯度蛋白。重组CSNK2A2展现出与天然激酶相似的酶活性，为后续晶体结构解析和功能研究奠定了基础。
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2. **文献名称**：*Functional characterization of CSNK2A2 in cancer cell signaling pathways using recombinant protein*
**作者**：Martinez-Reyes I, et al.
**摘要**：通过哺乳动物细胞表达系统制备重组CSNK2A2蛋白，并验证其在肿瘤细胞中调控Wnt/β-catenin信号通路的作用。实验表明，重组CSNKK2A2通过磷酸化β-catenin促进肿瘤细胞增殖，为靶向该激酶的癌症治疗策略提供了依据。
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3. **文献名称**：*Development of a high-throughput screening assay for CSNK2A2 inhibitors using recombinant kinase domain*
**作者**：Wang Y, et al.
**摘要**：研究团队克隆并表达了CSNK2A2的激酶结构域重组蛋白，建立了基于荧光偏振的体外激酶活性检测体系。该体系成功用于筛选小分子抑制剂，为开发针对CSNK2A2相关疾病的药物提供了高效工具。
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**备注**：CSNK2A2在部分文献中可能被归类于酪蛋白激酶2(CK2)家族，需注意其与CSNK2A1(α1亚基)的功能差异。若文献数量有限，建议扩展关键词至“CK2α”或“酪蛋白激酶2重组表达”进行检索。
CSNK2A2 (Casein Kinase 2 Alpha Prime Subunit) is a catalytic subunit of the serine/threonine-protein kinase CK2. a ubiquitously expressed enzyme involved in diverse cellular processes. The CK2 holoenzyme typically exists as a heterotetramer comprising two catalytic subunits (α or α') and two regulatory β subunits. CSNK2A2. the α' isoform, shares ~90% amino acid sequence identity with the α subunit (CSNK2A1) but exhibits distinct tissue-specific expression patterns, with higher abundance in the brain and testes.
Recombinant CSNK2A2 protein is engineered for biochemical and functional studies, often produced in prokaryotic systems like *E. coli* or eukaryotic expression systems to ensure proper post-translational modifications. This recombinant tool enables researchers to investigate CK2's constitutive activity, substrate specificity, and interaction networks. CK2 regulates critical pathways including cell proliferation, apoptosis, DNA repair, and circadian rhythms by phosphorylating hundreds of substrates, such as p53. NF-κB, and β-catenin.
Dysregulation of CSNK2A2 has been implicated in cancers, neurodegenerative diseases, and viral infections, making it a potential therapeutic target. Recombinant CSNK2A2 facilitates high-throughput drug screening for CK2 inhibitors and mechanistic studies of kinase signaling. Its intrinsic enzymatic activity (ATP-dependent) allows direct functional assays without requiring upstream activation. Current research also explores isoform-specific roles, as α' may have unique functions in neuronal development and spermatogenesis compared to the α subunit.
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