| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSNK2A1 |
| Uniprot No | P68400 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-391aa |
| 氨基酸序列 | MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ |
| 预测分子量 | 52.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSNK2A1重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*Expression and Purification of Recombinant Human CSNK2A1 in Escherichia coli*
**作者**：Litchfield DW, et al.
**摘要**：该研究报道了利用大肠杆菌表达系统高效表达并纯化具有催化活性的人源CSNK2A1重组蛋白，优化了蛋白可溶性和酶活性的条件。
2. **文献名称**：*Structural Insights into CSNK2A1 Kinase Regulation by ATP-Competitive Inhibitors*
**作者**：Sarno S, Pinna LA.
**摘要**：通过X射线晶体学解析了重组CSNKK2A1蛋白与ATP类似物结合的复合物结构，揭示了其催化口袋的构象变化及抑制剂选择性机制。
3. **文献名称**：*CSNK2A1-Mediated Phosphorylation of p53 Modulates Autophagy in Cancer Cells*
**作者**：Guerra B, Issinger OG.
**摘要**：利用重组CSNK2A1蛋白进行体外磷酸化实验，证明其通过磷酸化p53蛋白调控肿瘤细胞自噬过程，为靶向激酶治疗提供了理论依据。
注：以上文献为示例性概括，实际引用需根据具体研究内容查询PubMed或Web of Science等数据库获取。
Casein kinase 2 alpha 1 (CSNK2A1) is a catalytic subunit of the highly conserved serine/threonine-protein kinase CK2 complex, which plays a critical role in regulating cellular processes such as proliferation, apoptosis, and DNA repair. The CSNK2A1 gene encodes a 42-45 kDa protein containing a kinase domain responsible for phosphorylating diverse substrates, including transcription factors, signaling molecules, and chromatin-associated proteins. Dysregulation of CK2 activity, often linked to CSNK2A1 overexpression, has been implicated in cancer, neurodegenerative diseases, and inflammatory conditions.
Recombinant CSNK2A1 protein is engineered using expression systems like *E. coli* or mammalian cells to produce functional kinase for research and therapeutic development. Its recombinant form typically retains enzymatic activity, enabling studies on substrate interactions, kinase inhibition, and signaling pathway dissection. Purification tags (e.g., His-tag) are commonly added to facilitate isolation. Researchers utilize this tool to investigate CK2's oncogenic potential, as it promotes tumor growth by enhancing survival pathways (e.g., PI3K/AKT) and stabilizing oncoproteins. Additionally, recombinant CSNK2A1 aids in drug discovery, serving as a target for small-molecule inhibitors aimed at treating CK2-driven malignancies. Its conserved structure across species also supports cross-model mechanistic studies. Despite its pathogenic associations, CK2's essential role in normal cellular homeostasis underscores the need for precise targeting strategies in therapeutic applications.
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