| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSNK1D |
| Uniprot No | P48730 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-415aa |
| 氨基酸序列 | MELRVGNRYRLGRKIGSGSFGDIYLGTDIAAGEEVAIKLECVKTKHPQLHIESKIYKMMQGGVGIPTIRWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFATYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGASRAADDAERERRDREERLRHSRNPATRGLPSTASGRLRGTQEVAPPTPLTPTSHTANTSPRPVSGMERERKVSMRLHRGAPVNISSSDLTGRQDTSRMSTSQIPGRVASSGLQSVVHR |
| 预测分子量 | 54.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSNK1D重组蛋白的3篇代表性文献示例(注：文献信息为模拟生成，仅供参考)：
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1. **文献名称**: "Recombinant expression and functional characterization of human CSNK1D in circadian rhythm regulation"
**作者**: Zhang L, et al.
**摘要**: 研究报道了通过大肠杆菌表达系统成功制备重组人源CSNK1D蛋白，并验证其磷酸化核心时钟蛋白PER2的能力，揭示了其在哺乳动物昼夜节钟调控中的关键作用。
2. **文献名称**: "Structural insights into CSNK1D kinase activity via recombinant protein crystallography"
**作者**: Müller S, et al.
**摘要**: 利用昆虫细胞系统表达并纯化高活性CSNK1D重组蛋白，通过X射线晶体学解析其三维结构，阐明了ATP结合口袋及底物识别机制，为靶向抑制剂设计提供依据。
3. **文献名称**: "CSNK1D recombinant protein enhances β-catenin signaling in colorectal cancer models"
**作者**: Kim J, Park H.
**摘要**: 研究在HEK293细胞中表达带有标签的CSNK1D重组蛋白，证实其通过磷酸化DVL2蛋白激活Wnt/β-catenin通路，促进结直肠癌细胞增殖及转移潜能。
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如需真实文献，建议在PubMed或Web of Science中检索关键词“CSNK1D recombinant protein”或结合具体研究方向筛选。
CSNK1D (Casein Kinase 1 Delta) is a serine/threonine protein kinase belonging to the highly conserved casein kinase 1 (CK1) family. It plays a critical role in regulating diverse cellular processes, including circadian rhythms, DNA repair, cell cycle progression, and Wnt signaling. The enzyme phosphorylates target substrates by recognizing specific motifs, often in a context-dependent manner, and its activity is modulated by post-translational modifications or interaction with regulatory proteins. Dysregulation of CSNK1D has been implicated in various diseases, including cancer, neurodegenerative disorders, and sleep-related pathologies, making it a potential therapeutic target.
Recombinant CSNK1D protein is produced using biotechnological methods, typically through heterologous expression in systems like Escherichia coli, insect cells, or mammalian cell cultures. This engineered protein retains the kinase domain's functional integrity, enabling researchers to study its enzymatic activity, substrate specificity, and inhibitor interactions in controlled in vitro settings. Purification tags (e.g., GST, His-tag) are often fused to facilitate isolation and detection.
In research, recombinant CSNK1D serves as a vital tool for high-throughput drug screening, structural studies (e.g., crystallography), and mechanistic investigations of phosphorylation cascades. It has been particularly valuable in developing selective inhibitors for cancer therapy, given its role in stabilizing oncoproteins like β-catenin. Recent studies also explore its involvement in circadian clock regulation, offering insights into metabolic disorders. The availability of active recombinant CSNK1D accelerates both basic research and translational applications in precision medicine.
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