| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSNK1A1 |
| Uniprot No | P48729 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-337aa |
| 氨基酸序列 | ASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF |
| 预测分子量 | 42.8kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSNK1A1重组蛋白的3篇参考文献示例(文献信息为虚构，仅供格式参考)：
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1. **标题**: "Expression and Purification of Recombinant Human CSNK1A1 in Escherichia coli for Biochemical Studies"
**作者**: Zhang, L., et al.
**摘要**: 该研究描述了在大肠杆菌系统中高效表达和纯化重组人源CSNK1A1蛋白的优化方法。作者通过亲和层析和尺寸排阻色谱获得高纯度蛋白，并验证了其激酶活性，为体外酶学实验提供了可靠工具。
2. **标题**: "Structural Insights into CSNK1A1 Kinase Regulation by Phosphorylation"
**作者**: Müller, R., et al.
**摘要**: 通过X射线晶体学解析了重组CSNK1A1的磷酸化状态结构，揭示了其自抑制机制及底物结合位点的构象变化，阐明了翻译后修饰对其激酶活性的调控机制。
3. **标题**: "CSNK1A1 Recombinant Protein as a Target for Wnt Signaling Inhibitors"
**作者**: Tanaka, K., et al.
**摘要**: 利用重组CSNK1A1蛋白进行高通量药物筛选，发现新型小分子抑制剂可特异性阻断其与β-catenin的相互作用，为治疗Wnt信号通路异常激活的癌症提供了潜在策略。
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*注：以上文献为示例性质，实际引用时请查询真实数据库(如PubMed)获取准确信息。*
CSNK1A1 (Casein Kinase 1 Alpha 1) is a serine/threonine-protein kinase belonging to the casein kinase 1 (CK1) family, which plays critical roles in regulating diverse cellular processes, including signal transduction, cell cycle progression, DNA repair, and circadian rhythms. This enzyme is ubiquitously expressed in eukaryotic organisms and is evolutionarily conserved, highlighting its fundamental biological importance. The CSNK1A1 gene encodes a protein of approximately 337 amino acids, characterized by a conserved kinase domain that mediates phosphorylation of substrate proteins. Its activity is modulated by autophosphorylation and interactions with regulatory proteins or signaling pathways, such as Wnt/β-catenin and p53 pathways.
Recombinant CSNK1A1 protein is produced in vitro using expression systems like *E. coli* or mammalian cells, enabling large-scale purification for biochemical and functional studies. This engineered protein retains enzymatic activity and is widely used to investigate kinase-substrate interactions, screen kinase inhibitors, and study mechanisms underlying diseases linked to CK1 dysregulation. For instance, aberrant CSNK1A1 activity has been implicated in cancers, neurodegenerative disorders (e.g., Alzheimer’s disease), and developmental defects. Researchers also employ recombinant CSNK1A1 to explore its role in phosphorylating key targets such as β-catenin, DVL proteins, and circadian clock components. Structural studies using recombinant variants have further elucidated its catalytic mechanisms and guided drug development. Despite its broad functionality, CSNK1A1's context-dependent regulation and isoform-specific effects remain active areas of investigation, underscoring its complexity in cellular signaling networks.
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