| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSF2RA |
| Uniprot No | P15509 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-320aa |
| 氨基酸序列 | ADPLIPEKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTDKK NRVVEPRLSNNECSCTFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGR EGTAAQNFSCFIYNADLMNCTWARGPTAPRDVQYFLYIRNSKRRREIRCP YYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREIGIQFFDSLLDTKKIE RFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKNTQPGT ENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEFG SDDGHHHHHH |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSF2RA重组蛋白的3篇参考文献，简要整理如下：
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1. **文献名称**：*"Human granulocyte-macrophage colony-stimulating factor receptor alpha chain gene: structure, chromosomal localization, and regulation of expression"*
**作者**：Hansen, G., et al.
**摘要**：该研究首次克隆并鉴定了人CSF2RA基因，揭示了其染色体定位(X/Y假常染色体区)，并证明其编码的受体α链与GM-CSF结合，为后续重组蛋白表达提供了分子基础。
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2. **文献名称**：*"GM-CSF receptor alpha deficiency in the lungs exacerbates surfactant accumulation via impaired macrophage maturation"*
**作者**：Sakagami, T., et al.
**摘要**：通过构建CSF2RA缺陷小鼠模型，研究发现重组CSF2RA蛋白可恢复巨噬细胞对肺泡表面活性物质的清除能力，表明其在肺部稳态中的关键作用及潜在治疗价值。
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3. **文献名称**：*"Functional characterization of a novel GM-CSF receptor alpha variant with altered signaling capacity"*
**作者**：Williams, L., et al.
**摘要**：通过重组表达不同CSF2RA突变体，研究发现受体α链的结构修饰可调控JAK/STAT信号通路活性，为靶向GM-CSF受体的药物设计提供理论依据。
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4. **文献名称**：*"Engineering a soluble high-affinity GM-CSF receptor alpha for therapeutic applications"*
**作者**：Hercus, T.R., et al.
**摘要**：报道了一种通过蛋白质工程优化的可溶性CSF2RA重组蛋白，其显著提高对GM-CSF的亲和力，并验证其在抑制自身免疫性疾病模型中的疗效。
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注：以上文献为模拟示例，实际引用时需核对具体出版物信息及准确性。建议通过PubMed或Web of Science以“CSF2RA recombinant protein”为关键词检索最新研究。
The CSF2RA (Colony Stimulating Factor 2 Receptor Subunit Alpha) gene encodes the alpha subunit of the granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor, a critical cytokine receptor involved in hematopoiesis and immune regulation. Located on the X/Y pseudoautosomal region, CSF2RA shares homology across species and forms a heterodimeric receptor complex with the beta subunit (CSF2RB) to mediate GM-CSF signaling. GM-CSF binding triggers JAK/STAT, MAPK, and PI3K pathways, regulating survival, proliferation, and differentiation of myeloid cells, including macrophages, neutrophils, and dendritic cells.
Mutations in CSF2RA are linked to hereditary pulmonary alveolar proteinosis (hPAP), a rare lung disorder characterized by surfactant accumulation due to impaired macrophage function. This association underscores the receptor's role in alveolar homeostasis. Recombinant CSF2RA protein, typically produced in mammalian expression systems to ensure proper glycosylation and folding, serves as a vital tool for studying GM-CSF receptor biology. It enables researchers to investigate ligand-receptor interactions, signal transduction mechanisms, and pathological pathways in diseases like hPAP or autoimmune conditions. Additionally, recombinant CSF2RA has therapeutic potential, such as in receptor replacement strategies or as a decoy to modulate excessive GM-CSF activity in inflammatory disorders. Its development supports targeted drug screening and biomarker studies, bridging basic research with clinical applications in immunology and hematology.
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