| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SERPINB4 |
| Uniprot No | P48594 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-390aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMNSLSEANTKFMFDLFQQFRKSKENNI FYSPISITSALGMVLLGAKDNTAQQISKVLHFDQVTENTTEKAATYHVDR SGNVHHQFQKLLTEFNKSTDAYELKIANKLFGEKTYQFLQEYLDAIKKFY QTSVESTDFANAPEESRKKINSWVESQTNEKIKNLFPDGTIGNDTTLVLV NAIYFKGQWENKFKKENTKEEKFWPNKNTYKSVQMMRQYNSFNFALLEDV QAKVLEIPYKGKDLSMIVLLPNEIDGLQKLEEKLTAEKLMEWTSLQNMRE TCVDLHLPRFKMEESYDLKDTLRTMGMVNIFNGDADLSGMTWSHGLSVSK VLHKAFVEVTEEGVEAAAATAVVVVELSSPSTNEEFCCNHPFLFFIRQNK TNSILFYGRFSSP |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SERPINB4重组蛋白的参考文献示例，基于学术知识整理而成：
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1. **标题**：*Recombinant SERPINB4 inhibits cathepsin G activity and modulates inflammation in psoriasis*
**作者**：A. Smith et al.
**摘要**：该研究通过体外实验证实，重组SERPINB4蛋白可有效抑制中性粒细胞分泌的组织蛋白酶G，并减少银屑病模型中促炎细胞因子的释放，提示其在调节皮肤炎症中的潜在作用。
2. **标题**：*Functional characterization of recombinant SERPINB4 in squamous cell carcinoma progression*
**作者**：Y. Zhang et al.
**摘要**：研究团队表达并纯化了重组SERPINB4蛋白，发现其通过抑制蛋白酶活性促进鳞状细胞癌细胞的抗凋亡能力，并与患者预后不良相关。
3. **标题**：*Development of a SERPINB4-based ELISA assay using recombinant protein for cancer biomarker screening*
**作者**：J. Lee et al.
**摘要**：利用重组SERPINB4蛋白开发高灵敏度ELISA检测方法，验证了其在头颈部鳞癌患者血清中的高表达，为无创诊断提供新策略。
4. **标题**：*Structural insights into SERPINB4’s protease inhibition mechanism via recombinant protein crystallography*
**作者**：C. Müller et al.
**摘要**：通过重组SERPINB4蛋白的晶体结构解析，揭示了其与靶蛋白酶相互作用的特异性位点，为设计相关疾病治疗药物提供结构基础。
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**注**：以上文献信息为示例性质，具体内容请以实际发表的学术论文为准。建议通过PubMed或Web of Science等平台检索最新研究。
SERPINB4. a member of the serine protease inhibitor (SERPIN) superfamily, belongs to the clade B subfamily predominantly expressed in epithelial and immune cells. Also known as squamous cell carcinoma antigen 2 (SCCA2), it is encoded by the SERPINB4 gene located on human chromosome 18q21.3. This 44-kDa protein functions as a cross-class inhibitor, targeting serine proteases like cathepsin G and chymase, as well as the cysteine protease cathepsin K, through its reactive center loop (RCL) mechanism. Its inhibitory activity plays roles in regulating inflammation, tissue remodeling, and protease-mediated signaling pathways.
Recombinant SERPINB4 is engineered using expression systems (e.g., E. coli or mammalian cells) to produce purified protein for research and therapeutic applications. Structurally, it retains the conserved SERPIN fold but shows distinct substrate specificity compared to its paralog SERPINB3. Biologically, SERPINB4 is upregulated in Th2-dominant inflammatory conditions, including atopic dermatitis, psoriasis, and allergic asthma, where it modulates immune cell activity and protease balance. In oncology, it exhibits dual roles: overexpression in squamous cell carcinomas correlates with tumor progression and chemotherapy resistance, potentially through anti-apoptotic effects, while some studies suggest metastasis-suppressive functions in specific contexts.
Current research explores its utility as a disease biomarker and therapeutic target. Recombinant SERPINB4 serves as a tool to study protease networks in chronic inflammation and cancer microenvironments, with emerging interest in engineered variants for targeted protease inhibition. Challenges include elucidating context-dependent functional switches and optimizing delivery strategies for therapeutic applications.
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