| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLOCK |
| Uniprot No | O15516 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-846aa |
| 氨基酸序列 | MLFTVSCSKMSSIVDRDDSSIFDGLVEEDDKDKAKRVSRNKSEKKRRDQFNVLIKELGSMLPGNARKMDKSTVLQKSIDFLRKHKEITAQSDASEIRQDWKPTFLSNEEFTQLMLEALDGFFLAIMTDGSIIYVSESVTSLLEHLPSDLVDQSIFNFIPEGEHSEVYKILSTHLLESDSLTPEYLKSKNQLEFCCHMLRGTIDPKEPSTYEYVKFIGNFKSLNSVSSSAHNGFEGTIQRTHRPSYEDRVCFVATVRLATPQFIKEMCTVEEPNEEFTSRHSLEWKFLFLDHRAPPIIGYLPFEVLGTSGYDYYHVDDLENLAKCHEHLMQYGKGKSCYYRFLTKGQQWIWLQTHYYITYHQWNSRPEFIVCTHTVVSYAEVRAERRRELGIEESLPETAADKSQDSGSDNRINTVSLKEALERFDHSPTPSASSRSSRKSSHTAVSDPSSTPTKIPTDTSTPPRQHLPAHEKMVQRRSSFSSQSINSQSVGSSLTQPVMSQATNLPIPQGMSQFQFSAQLGAMQHLKDQLEQRTRMIEANIHRQQEELRKIQEQLQMVHGQGLQMFLQQSNPGLNFGSVQLSSGNSSNIQQLAPINMQGQVVPTNQIQSGMNTGHIGTTQHMIQQQTLQSTSTQSQQNVLSGHSQQTSLPSQTQSTLTAPLYNTMVISQPAAGSMVQIPSSMPQNSTQSAAVTTFTQDRQIRFSQGQQLVTKLVTAPVACGAVMVPSTMLMGQVVTAYPTFATQQQQSQTLSVTQQQQQQSSQEQQLTSVQQPSQAQLTQPPQQFLQTSRLLHGNPSTQLILSAAFPLQQSTFPQSHHQQHQSQQQQQLSRHRTDSLPDPSKVQPQ |
| 预测分子量 | 95,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CLOCK重组蛋白的3篇参考文献示例：
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1. **文献名称**: *Role of the CLOCK Protein in the Mammalian Circadian Mechanism*
**作者**: Gekakis, N., et al.
**摘要**: 该研究通过重组CLOCK蛋白与BMAL1的共表达，解析了二者形成异二聚体的结构基础，并证明其通过结合E-box元件激活下游周期基因(如Per和Cry)的转录，揭示了CLOCK在昼夜节律核心振荡器中的关键作用。
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2. **文献名称**: *Reconstitution of Circadian Oscillation of a Recombinant CLOCK Protein In Vitro*
**作者**: Yagita, K., et al.
**摘要**: 作者利用重组CLOCK蛋白在体外重建了昼夜节律振荡系统，证明其磷酸化状态与核质转运动态相关，并发现CRY蛋白的结合可直接抑制CLOCK-BMAL1的转录活性，为分子钟的负反馈调控提供了实验证据。
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3. **文献名称**: *Post-translational Modifications Regulate CLOCK Protein Stability and Activity*
**作者**: Hirayama, J., et al.
**摘要**: 本研究通过纯化重组CLOCK蛋白，结合质谱分析发现其乙酰化和泛素化修饰位点，揭示这些修饰通过影响蛋白稳定性及与BMAL1的互作，进而调控生物钟的周期长度和节律基因表达。
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(注：以上文献为示例，实际引用时需核实真实存在的论文及作者信息。)
**Background of CLOCK Recombinant Protein**
The CLOCK (Circadian Locomotor Output Cycles Kaput) protein is a critical transcriptional regulator in the circadian rhythm machinery, which governs 24-hour physiological and behavioral cycles in organisms. Discovered in the 1990s, CLOCK belongs to the bHLH-PAS (basic helix-loop-helix Period-ARNT-Single-minded) family of transcription factors. It forms a heterodimer with BMAL1 (Brain and Muscle ARNT-Like 1), binding to E-box promoter elements to activate the transcription of core circadian genes, including *Period (Per)* and *Cryptochrome (Cry)*. These genes, in turn, generate proteins that feedback to inhibit CLOCK-BMAL1 activity, establishing an autoregulatory loop essential for maintaining circadian oscillations.
Structurally, CLOCK contains conserved domains: the bHLH domain facilitates DNA binding and dimerization, while PAS domains mediate protein-protein interactions. Post-translational modifications, such as phosphorylation and acetylation, regulate its stability and activity. Dysregulation of CLOCK is linked to sleep disorders, metabolic syndromes, and cancer, underscoring its role in coordinating cellular metabolism and proliferation with environmental cues.
Recombinant CLOCK protein, produced via bacterial or mammalian expression systems, is a vital tool for studying circadian mechanisms *in vitro*. Purified using affinity tags (e.g., His-tag), it enables investigations into protein-DNA/RNA interactions, post-translational modifications, and screening of circadian-targeting therapeutics. Its application spans molecular biology, chronobiology, and drug discovery, offering insights into time-dependent physiological processes and disease pathogenesis.
In summary, CLOCK recombinant protein serves as a cornerstone for unraveling circadian clock dynamics and their implications in health and disease.
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