| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HTRA4 |
| Uniprot No | P83105 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 159-376aa |
| 氨基酸序列 | SAGPLRRNYNFIAAVVEKVAPSVVHVQLWGRLLHGSRLVPVYSGSGFIVS EDGLIITNAHVVRNQQWIEVVLQNGARYEAVVKDIDLKLDLAVIKIESNA ELPVLMLGRSSDLRAGEFVVALGSPFSLQNTATAGIVSTKQRGGKELGMK DSDMDYVQIDATINYGNSGGPLVNLDGDVIGVNSLRVTDGISFAIPSDRV RQFLAEYHEHQMKGKAFS |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HTRA4重组蛋白的3篇参考文献示例(文献信息为模拟生成，仅供示例参考)：
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1. **文献名称**：*Recombinant HTRA4 Expression and Its Role in Trophoblast Function During Preeclampsia*
**作者**：Chen L, et al.
**摘要**：本研究通过在大肠杆菌系统中表达并纯化重组HTRA4蛋白，分析其在胎盘滋养层细胞中的蛋白酶活性及对细胞迁移的影响。结果表明，HTRA4可能通过调节细胞外基质重塑参与先兆子痫的病理过程。
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2. **文献名称**：*Structural Characterization of Recombinant HTRA4 and Its Interaction with Pregnancy-Specific Glycoproteins*
**作者**：Wang Y, et al.
**摘要**：利用昆虫细胞表达系统制备重组HTRA4蛋白，并通过X射线晶体学解析其三维结构。研究发现，HTRA4与妊娠特异性糖蛋白(PSG)的结合可能调控母胎界面免疫耐受，为妊娠相关疾病提供机制见解。
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3. **文献名称**：*Functional Analysis of HTRA4 Mutants Using Recombinant Protein Assays in Ocular Disorders*
**作者**：Smith J, et al.
**摘要**：通过哺乳动物细胞表达体系生成野生型和突变型HTRA4重组蛋白，研究其蛋白酶活性与年龄相关性黄斑变性(AMD)的关联。实验表明，HTRA4突变体可能导致异常ECM降解，参与AMD发病。
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**注意**：以上文献信息为示例，实际研究中建议通过PubMed、Google Scholar或Web of Science等平台检索最新实证论文(关键词如“HTRA4 recombinant protein”或“HTRA4 expression and purification”)。真实文献需核对作者、期刊及具体研究内容。
**Background of HTRA4 Recombinant Protein**
HTRA4 (HtrA Serine Peptidase 4) is a member of the evolutionarily conserved HtrA family of serine proteases, characterized by a trypsin-like protease domain and a PDZ domain involved in protein-protein interactions. Unlike other HtrA members (e.g., HTRA1. HTRA2), HTRA4 is predominantly expressed in placental tissues, where it plays a critical role in maintaining placental development and function. It regulates extracellular matrix (ECM) remodeling, cellular stress responses, and trophoblast invasion—a process essential for proper embryo implantation and placental vascularization. Dysregulation of HTRA4 has been linked to pregnancy-related disorders, such as pre-eclampsia and intrauterine growth restriction, due to its impact on trophoblast behavior and placental angiogenesis.
Recombinant HTRA4 protein is produced using biotechnological methods, often via expression systems like *E. coli*, mammalian cells, or insect cells, followed by purification to ensure high specificity and activity. This engineered protein retains the enzymatic function of native HTRA4. enabling researchers to study its protease activity, substrate interactions, and regulatory mechanisms *in vitro*.
In research, HTRA4 recombinant protein serves as a vital tool for investigating its biological roles in placental biology and pregnancy pathologies. It is also explored for therapeutic potential, such as targeting HTRA4 in disease models or developing diagnostic biomarkers. Additionally, studies examine its involvement in cancer progression, as aberrant serine protease activity may influence tumor invasion and metastasis. Overall, HTRA4 recombinant protein bridges mechanistic insights into developmental and disease processes, offering avenues for translational applications.
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